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Title: Rabbit ubiquitin-activating enzyme E1: cDNA cloning, sequence and expression
Authors: Sun, B.
Jeyaseelan, K. 
Chung, M.C.M.
Teo, T.-S. 
Keywords: Protein degradation
Tissue distribution
Issue Date: 1997
Citation: Sun, B., Jeyaseelan, K., Chung, M.C.M., Teo, T.-S. (1997). Rabbit ubiquitin-activating enzyme E1: cDNA cloning, sequence and expression. Gene 196 (1-2) : 19-23. ScholarBank@NUS Repository.
Abstract: A cDNA clone encoding ubiquitin-activating enzyme E1 has been isolated from a rabbit heart cDNA library and sequenced. The 3.485 kb cDNA contains an open reading frame of 1058 amino acid residues which predicts a protein of approx. 118 kDa. The deduced protein sequence exhibits a very high homology to other ubiquitin-activating enzymes identified in a variety of organisms. Northern blot analysis reveals a single transcript of apporx. 3.5 kb in all the rabbit tissues examined. The entire coding region of the rabbit E1 cDNA has been expressed as a his-tagged protein. The recombinant protein has been verified by its ability to cross-react with anti-human E1 antibodies. Ubiquitin thiolester assay shows that the recombinant rabbit E1 protein is functional.
Source Title: Gene
ISSN: 03781119
DOI: 10.1016/S0378-1119(97)00154-6
Appears in Collections:Staff Publications

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