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|Title:||Rabbit ubiquitin-activating enzyme E1: cDNA cloning, sequence and expression||Authors:||Sun, B.
|Issue Date:||1997||Citation:||Sun, B., Jeyaseelan, K., Chung, M.C.M., Teo, T.-S. (1997). Rabbit ubiquitin-activating enzyme E1: cDNA cloning, sequence and expression. Gene 196 (1-2) : 19-23. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1119(97)00154-6||Abstract:||A cDNA clone encoding ubiquitin-activating enzyme E1 has been isolated from a rabbit heart cDNA library and sequenced. The 3.485 kb cDNA contains an open reading frame of 1058 amino acid residues which predicts a protein of approx. 118 kDa. The deduced protein sequence exhibits a very high homology to other ubiquitin-activating enzymes identified in a variety of organisms. Northern blot analysis reveals a single transcript of apporx. 3.5 kb in all the rabbit tissues examined. The entire coding region of the rabbit E1 cDNA has been expressed as a his-tagged protein. The recombinant protein has been verified by its ability to cross-react with anti-human E1 antibodies. Ubiquitin thiolester assay shows that the recombinant rabbit E1 protein is functional.||Source Title:||Gene||URI:||http://scholarbank.nus.edu.sg/handle/10635/38154||ISSN:||03781119||DOI:||10.1016/S0378-1119(97)00154-6|
|Appears in Collections:||Staff Publications|
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