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|Title:||A genomic region encoding stonefish (Synanceja horrida) stonustoxin beta-subunit contains an intron||Authors:||Ghadessy, F.J.
|Issue Date:||1994||Citation:||Ghadessy, F.J., Jeyaseelan, K., Chung, M.C.M., Khoo, H.E., Yuen, R. (1994). A genomic region encoding stonefish (Synanceja horrida) stonustoxin beta-subunit contains an intron. Toxicon 32 (12) : 1684-1688. ScholarBank@NUS Repository. https://doi.org/10.1016/0041-0101(94)90329-8||Abstract:||The polymerase chain reaction (PCR) has been used to amplify a 1899 base pair fragment from stonefish genomic DNA. A comparison of the translated nucleotide sequence of this product with the separately determined N-terminal amino acid sequence of the β-subunit reveals the presence of a 416 bp intron at Gly 18. The nucleotide sequence following this intron encodes 476 amino acids whose sequence showed no homology to other known toxins. This region, however, contained amino acid sequences identical to internal peptide sequences determined separately from the toxin's β-subunit.||Source Title:||Toxicon||URI:||http://scholarbank.nus.edu.sg/handle/10635/38151||ISSN:||00410101||DOI:||10.1016/0041-0101(94)90329-8|
|Appears in Collections:||Staff Publications|
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