Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0006-291X(02)02018-1
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dc.titleLoss of oxidized and chlorinated bases in DNA treated with reactive oxygen species: Implications for assessment of oxidative damage in vivo
dc.contributor.authorWhiteman, M.
dc.contributor.authorHong, H.S.
dc.contributor.authorJenner, A.
dc.contributor.authorHalliwell, B.
dc.date.accessioned2013-06-05T09:46:08Z
dc.date.available2013-06-05T09:46:08Z
dc.date.issued2002
dc.identifier.citationWhiteman, M., Hong, H.S., Jenner, A., Halliwell, B. (2002). Loss of oxidized and chlorinated bases in DNA treated with reactive oxygen species: Implications for assessment of oxidative damage in vivo. Biochemical and Biophysical Research Communications 296 (4) : 883-889. ScholarBank@NUS Repository. https://doi.org/10.1016/S0006-291X(02)02018-1
dc.identifier.issn0006291X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/38112
dc.description.abstractOxidative damage to DNA has been reported to occur in a wide variety of disease states. The most widely used "marker" for oxidative DNA damage is 8-hydroxyguanine. However, the use of only one marker has limitations. Exposure of calf thymus DNA to an ·OH-generating system (CuCl2, ascorbate, H2O2) or to hypochlorous acid (HOCl), led to the extensive production of multiple oxidized or chlorinated DNA base products, as measured by gas chromatography-mass spectrometry. The addition of peroxynitrite (ONOO-) (<200 μM) or SIN-1 (1 mM) to oxidized DNA led to the extensive loss of 8-hydroxyguanine, 5-hydroxycytosine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine, 2-hydroxyadenine, 8-hydroxyadenine, and 4,6-diamino-5-formamidopyrimidine were lost at higher ONOO- concentrations (>200 μM). Exposure of DNA to HOCl led to the generation of 5-Cl uracil and 8-Cl adenine and addition of ONOO- (<200 μM) or SIN-1 (1 mM) led to an extensive loss of 8-Cl adenine and a small loss of 5-Cl uracil at higher concentrations (>500 μM). An ·OH-generating system (CuCl2/ascorbate/H2O2) could also destroy these chlorinated species. Treatment of oxidized or chlorinated DNA with acidified nitrite (NO2-, pH 3) led to substantial loss of various base lesions, in particular 8-OH guanine, 5-OH cytosine, thymine glycol, and 8-Cl adenine. Our data indicate the possibility that when ONOO-, nitrite in regions of low pH or ·OH are produced at sites of inflammation, levels of certain damaged DNA bases could represent an underestimate of ongoing DNA damage. This study emphasizes the need to examine more than one modified DNA base when assessing the role of reactive species in human disease. © 2002 Elsevier Science (USA). All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0006-291X(02)02018-1
dc.sourceScopus
dc.subject5-Chlorocytosine
dc.subject5-Chlorouracil
dc.subject8-Hydroxyguanine
dc.subjectHypochlorous acid
dc.subjectOxidative DNA damage
dc.subjectPeroxynitrite
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/S0006-291X(02)02018-1
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume296
dc.description.issue4
dc.description.page883-889
dc.description.codenBBRCA
dc.identifier.isiut000180887000016
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