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|Title:||A ROLE FOR RUNX3 IN INFLAMMATION-INDUCED EXPRESSION OF IL23A IN GASTRIC EPITHELIAL CELLS||Authors:||HOR YIT TENG||Keywords:||RUNX3,IL23A,Gastric,Epithelial, inflammation, infection||Issue Date:||24-Aug-2012||Citation:||HOR YIT TENG (2012-08-24). A ROLE FOR RUNX3 IN INFLAMMATION-INDUCED EXPRESSION OF IL23A IN GASTRIC EPITHELIAL CELLS. ScholarBank@NUS Repository.||Abstract:||In a previous expression microarray study, IL23A was identified as a putative target gene of RUNX3, a transcription factor and a prominent gastric tumour suppressor. IL23A encodes for the unique subunit of IL-23, a heterodimeric cytokine necessary for pathogen surveillance in the gut. Using reporter gene and promoter occupancy assays, together with ectopic expression and RNAi knockdown studies, IL23A was demonstrated to be a genuine target gene of RUNX3 in gastric epithelial cells. Furthermore, RUNX3 is a critical requirement for the synergistic induction of IL23A by TNF and Helicobacter pylori¿ two key inflammatory signals strongly linked to human gastric carcinogenesis. In the presence of these stimuli, IL23A is robustly expressed and secreted by gastric epithelial cells, albeit not in its normal heterodimeric form. Lastly, stimulation of human PBMC-derived T cells with IL23A-containing supernatant revealed functions for this protein in promoting T cell proliferation and IFN production. Together, these data indicate that RUNX3 functions as a tumour suppressor in part by modulating gastric inflammation and mucosal immunity.||URI:||http://scholarbank.nus.edu.sg/handle/10635/37724|
|Appears in Collections:||Ph.D Theses (Open)|
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