Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/37594
Title: | Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding | Authors: | MARIE VIVIAN WONG TZU YENN | Keywords: | ID2, inhibitor of dna-binding, HLH, loop, ion, crystal | Issue Date: | 25-Jul-2012 | Citation: | MARIE VIVIAN WONG TZU YENN (2012-07-25). Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding. ScholarBank@NUS Repository. | Abstract: | The ID proteins, a class of transcription regulators, were named for their role as inhibitors of DNA-binding and differentiation. They contained a helix-loop-helix (HLH) domain without a basic DNA-binding domain and functioned through dimerization with basic-HLH transcription factors to inactivate their DNA-binding abilities. ID2, a member of the ID family was cloned, expressed and purified using strategies to overcome the known instability of this protein both in vitro and in vivo. The crystal structure of ID2 was solved to 2.1 ? and showed for the first time, a loop ion that was previously unreported in HLH structures. Key residues based on the structural analysis of ID2 were identified and mutated to gauge their importance in the dimerization of the ID protein family through competitive electrophoretic mobility shift assays. | URI: | http://scholarbank.nus.edu.sg/handle/10635/37594 |
Appears in Collections: | Ph.D Theses (Open) |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
01WongVTYM.pdf | 3.65 MB | Adobe PDF | OPEN | None | View/Download | |
02WongVTYM.pdf | 1.64 MB | Adobe PDF | OPEN | None | View/Download | |
03WongVTYM.pdf | 1.15 MB | Adobe PDF | OPEN | None | View/Download | |
04WongVTYM.pdf | 926.67 kB | Adobe PDF | OPEN | None | View/Download | |
05WongVTYM.pdf | 5.86 MB | Adobe PDF | OPEN | None | View/Download | |
06WongVTYM.pdf | 566.42 kB | Adobe PDF | OPEN | None | View/Download | |
07WongVTYM.pdf | 945.96 kB | Adobe PDF | OPEN | None | View/Download | |
08WongVTYM.pdf | 2.1 MB | Adobe PDF | OPEN | None | View/Download | |
09WongVTYM.pdf | 5.2 MB | Adobe PDF | OPEN | None | View/Download |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.