The Role of downstream of kinase (DOK)-3 in Toll-like receptor signalling

Abstract

Toll-like receptors (TLR) are germ-line encoded pattern recognition receptors (PRR) that detect infectious agents specific signatures present on pathogens collectively referred to as pathogen associated molecular pattern (PAMP). LPS, an endotoxin and poly(I:C) which is a synthetic oligonucleotide that mimics double-stranded RNA (dsRNA) binds to TLR-4 and 3 respectively and activates the TIR-containing adaptor inducing interferon beta (TRIF)-dependent signal transduction cascade. Because Downstream of kinase (Dok)-1 and Dok2 were known to regulate TLR, we hypothesized that Dok3 may be involved in TLR regulation as well. We hence investigated if Dok3 is involved in TLR signalling in macrophages.