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Title: | AP-2? regulates estrogen receptor-mediated long-range chromatin interactions and gene transcription | Authors: | TAN SI KEE | Keywords: | AP-2γ, ERα, FoxA1, ChIA-PET, ChIP-Seq, Chromatin | Issue Date: | 16-Jul-2012 | Citation: | TAN SI KEE (2012-07-16). AP-2? regulates estrogen receptor-mediated long-range chromatin interactions and gene transcription. ScholarBank@NUS Repository. | Abstract: | Estrogen receptor alpha (ERalpha) is a key player in breast cancer progression. Recently, ERalpha cistrome and interactome were mapped in MCF-7 cells, revealing the importance of spatial chromatin organization in estrogen-mediated transcription. Our study revealed that ERalpha binding sites (ERBS) identified from Chromatin Interaction Analysis-Paired End DiTag (ChIA-PET) are enriched of AP-2 motifs. AP-2gamma, a transcription factor associated with breast cancer, binds to ERBS harboring AP-2 motifs in a ligand-independent manner. Perturbation of AP-2gamma expression impaired ERalpha DNA binding, long-range chromatin interactions and gene transcription. In our genome-wide analyses, a large number AP-2gamma and ERalpha binding events converge together across the genome. Majority of these shared regions are also occupied by a pioneer factor, FoxA1. Functional interplay between AP-2gamma and FoxA1 was further demonstrated. Finally, ERBS associated with long-range chromatin interactions preferentially co-localize with AP-2gamma and FoxA1. Together, our results suggest that AP-2gamma is a novel ERalpha collaborative factor. | URI: | http://scholarbank.nus.edu.sg/handle/10635/35854 |
Appears in Collections: | Ph.D Theses (Open) |
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