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Title: Regulation of T Cell Clonal Expansion
Keywords: T cell, clonal expansion, regulation
Issue Date: 19-Jan-2012
Citation: CHUA REN JIE, ISAAC (2012-01-19). Regulation of T Cell Clonal Expansion. ScholarBank@NUS Repository.
Abstract: Clonal expansion of antigen-specific CD8 and CD4 T cells in vivo has been shown to differ between the two T cells subsets. It has been previously reported by Foulds et. al. that CD8 T cells undergo extensive proliferation compared to CD4 T cells. We wanted to investigate why CD8 T cells proliferated more than CD4 T cells and used an in vitro system with naive CD8+ and CD4+ T cells sorted from naive C57/BL6 mice according to their expression of CD44low-int CD62L+ CD25- to carry out our study. We went on to investigate the effect of p27, a cell cycle inhibitor, on the proliferation of naive CD8 and CD4 T cells using p27 KO mice. We observed that CD4 proliferation was enhanced in p27KO mice, comparable to that of WT CD8 T cells. Interestingly, we also observed that loss of p27 significantly increased CD4 effector population (TEM ? CD44hi CD62Llow population). Our results indicate that in CD4 T cells, p27 might be a potential target that can be used to manipulate their proliferation kinetics. However, in depth studies to dissect and compare the different phases of the cell cycle between CD8 and CD4 T cells would be required.
Appears in Collections:Ph.D Theses (Open)

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