Please use this identifier to cite or link to this item:
Title: Improvements on Humanized NOD/SCID lL-2rg-/- Mice
Authors: LI YAN
Keywords: humanized mice, macrophage, promonocyte, Influenza, BCG
Issue Date: 16-Apr-2012
Citation: LI YAN (2012-04-16). Improvements on Humanized NOD/SCID lL-2rg-/- Mice. ScholarBank@NUS Repository.
Abstract: Humanized mice offer a platform to study human diseases in vivo. Although progress has been made to improve human cell engraftment, the limited number of HSCs from cord blood, and the poor reconstitution and function of human monocytes/macrophages still remain as major limitations in current NSG mice. To overcome these problems, we first developed a robust co-culture system wherein cord blood CD133+CD34+ cells were co-cultured with MSCs engineered to express Angptl5 in cytokine supplemented media. This method eliminates the variations among different units of cord blood and makes large-scale experiments possible. The poor engraftment and function of human macrophages in NSG mice were corrected by a single injection of plasmids encoding human M-CSF. With the engraftment of human tissue macrophages in NSG mice, we not only open a door for the study of human macrophages in vivo, but also make the humanized mice one step closer to clinical applications.
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
LiY.pdf9.76 MBAdobe PDF



Page view(s)

checked on May 22, 2019


checked on May 22, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.