EFFECT OF CO2 LASER AND 38% DIAMMINE SILVER FLUORIDE TREATMENT OF ENAMEL AND ROOT DEMINERALIZATION

Abstract

ABSTRACT: Objectives: CO2 laser and Diammine-Silver-Fluoride have separately been shown to inhibit demineralization. However, the combined effect of the two modalities in inhibiting demineralization of enamel and root has not been investigated yet. The purpose of this study is to evaluate the effect of CO2 laser combined with 38% DSF treatment on enamel and root demineralization. Methods: Eight windows (4 in enamel and 4 in root) approximately 3mm x 1mm size were created on fifteen sound extracted human premolars and were randomly assigned to Control (No Treatment), CO2 laser, DSF, DSF+CO2 laser treatment groups. 38% DSF was applied for 2 minutes and CO2 laser with a wavelength of 10.6µm, 50HZ repetition rate, 200µs pulse was used. A 3-day pH cycling scheme for artificial lesion formation and polarized light microscopy for measuring the lesion depth was performed. Factorial ANOVA was employed to test the main effects and interaction. Results: The mean lesion depth (in µm) for each group were 303.75±12.30 (Control), 224.08 ± 8.61(DSF), 175.22±4.10 (CO2 laser), 152.74±3.90 (DSF+CO2 laser) in enamel and 1261.90± 11.68 (Control), 814.85± 8.89(DSF), 935.45±8.42 (CO2 laser), 614.37±4.84 (DSF+CO2 laser) in root. CO2 Laser and DSF had a statistically significant effect on lesion depth in enamel and root (all p<0.001). The interaction between CO2 laser and DSF was significant in enamel (p<0.005) and root (p<0.001). Individual tooth structure had no statistically significant effect on lesion depth formation in both enamel and root. Conclusion: Combining with CO2 laser may double the cariostatic effect of DSF.