Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0306-4522(03)00525-6
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dc.titleGroup IIA secretory phospholipase A2stimulates exocytosis and neurotransmitter release in pheochromocytoma-12 cells and cultured rat hippocampal neurons
dc.contributor.authorWei, S.
dc.contributor.authorFong, C.W.
dc.contributor.authorHong, W.
dc.contributor.authorOng, W.Y.
dc.contributor.authorThwin, M.M.
dc.contributor.authorGopalakrishnakone, P.
dc.contributor.authorFarooqui, A.A.
dc.date.accessioned2012-06-08T09:30:48Z
dc.date.available2012-06-08T09:30:48Z
dc.date.issued2003
dc.identifier.citationWei, S., Fong, C.W., Hong, W., Ong, W.Y., Thwin, M.M., Gopalakrishnakone, P., Farooqui, A.A. (2003). Group IIA secretory phospholipase A2stimulates exocytosis and neurotransmitter release in pheochromocytoma-12 cells and cultured rat hippocampal neurons. Neuroscience 121 (4) : 891-898. ScholarBank@NUS Repository. https://doi.org/10.1016/S0306-4522(03)00525-6
dc.identifier.issn03064522
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/33893
dc.description.abstractRecent evidence shows that secretory phospholipase A2 (sPLA 2) may play a role in membrane fusion and fission, and may thus affect neurotransmission. The present study therefore aimed to elucidate the effects of sPLA2 on vesicle exocytosis. External application of group IIA sPLA2 (purified crotoxin subunit B or purified human synovial sPLA2) caused an immediate increase in exocytosis and neurotransmitter release in pheochromocytoma-12 (PC12) cells, detected by carbon fiber electrodes placed near the cells, or by changes in membrane capacitance of the cells. EGTA and a specific inhibitor of sPLA2 activity, 12-epi-scalaradial, abolished the increase in neurotransmitter release, indicating that the effect of sPLA2 was dependent on calcium and sPLA2 enzymatic activity. A similar increase in neurotransmitter release was also observed in hippocampal neurons after external application of sPLA2, as detected by changes in membrane capacitance of the neurons. In contrast to external application, internal application of sPLA2 to PC12 cells and neurons produced blockade of neurotransmitter release. Our recent studies showed high levels of sPLA 2 activity in the normal rat hippocampus, medulla oblongata and cerebral neocortex. The sPLA2 activity in the hippocampus was significantly increased, after kainate-induced neuronal injury. The observed effects of sPLA2 on neurotransmitter release in this study may therefore have a physiological, as well as a pathological role. © 2003 IBRO. Published by Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0306-4522(03)00525-6
dc.sourceScopus
dc.subject12-epi-scalaradial
dc.subjectAmperometric currents
dc.subjectMembrane capacitance
dc.subjectNeurotransmitter release
dc.subjectPatch clamp
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.departmentANATOMY
dc.description.doi10.1016/S0306-4522(03)00525-6
dc.description.sourcetitleNeuroscience
dc.description.volume121
dc.description.issue4
dc.description.page891-898
dc.description.codenNRSCD
dc.identifier.isiut000186469800009
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