Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0956-5663(02)00075-1
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dc.titleAn ISFET-based immunosensor for the detection of β-Bungarotoxin
dc.contributor.authorSelvanayagam, Z.E.
dc.contributor.authorGopalakrishnakone, P.
dc.contributor.authorNeuzil, P.
dc.contributor.authorSridhar, U.
dc.contributor.authorSingh, M.
dc.contributor.authorHo, L.C.
dc.date.accessioned2012-06-08T09:30:33Z
dc.date.available2012-06-08T09:30:33Z
dc.date.issued2002
dc.identifier.citationSelvanayagam, Z.E., Gopalakrishnakone, P., Neuzil, P., Sridhar, U., Singh, M., Ho, L.C. (2002). An ISFET-based immunosensor for the detection of β-Bungarotoxin. Biosensors and Bioelectronics 17 (9) : 821-826. ScholarBank@NUS Repository. https://doi.org/10.1016/S0956-5663(02)00075-1
dc.identifier.issn09565663
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/33878
dc.description.abstractAn ion-sensitive field effect transistor (ISFET)-based immunosensor was developed to detect/quantitate β-Bungarotoxin (β-BuTx), a potent presynaptic neurotoxin from the venom of Bungarus multicinctus. A murine monoclonal antibody (mAb 15) specific to β-BuTx was immobilized onto silicon nitride wafers after silanization and activation with glutaraldehyde. A chip based enzyme linked-immunosorbantassay (ELISA) was performed to ascertain antigen binding to the immobilized antibody. To develop an electrochemical immunosensing system for the detection/quantitation of β-BuTx, an ISFET was used as a solid phase detector. MAb 15 was immobilized on the gate region of the ISFET. The antigen antibody reaction was monitored by the addition of urease conjugated rabbit anti-β-BuTx antibodies. The sensor can detect toxin level as low as 15.6 ng/ml. The efficacy of the sensor for the determination of β-BuTx from B. multicinctus venom was demonstrated in mouse model. Toxin concentration was highest at the site of injection (748.0±26 ng/ml) and moderate amount was found in the plasma (158.5±13 ng/ml). © 2002 Elsevier Science B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0956-5663(02)00075-1
dc.sourceScopus
dc.subjectβ-Bungarotoxin
dc.subjectDiagnosis of snake envenomation
dc.subjectImmunosensor
dc.subjectIon-sensitive field effect transistor (ISFET)
dc.typeArticle
dc.contributor.departmentANATOMY
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/S0956-5663(02)00075-1
dc.description.sourcetitleBiosensors and Bioelectronics
dc.description.volume17
dc.description.issue9
dc.description.page821-826
dc.description.codenBBIOE
dc.identifier.isiut000177919500013
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