Please use this identifier to cite or link to this item:
Title: An ISFET-based immunosensor for the detection of β-Bungarotoxin
Authors: Selvanayagam, Z.E. 
Gopalakrishnakone, P. 
Neuzil, P.
Sridhar, U.
Singh, M. 
Ho, L.C.
Keywords: β-Bungarotoxin
Diagnosis of snake envenomation
Ion-sensitive field effect transistor (ISFET)
Issue Date: 2002
Citation: Selvanayagam, Z.E., Gopalakrishnakone, P., Neuzil, P., Sridhar, U., Singh, M., Ho, L.C. (2002). An ISFET-based immunosensor for the detection of β-Bungarotoxin. Biosensors and Bioelectronics 17 (9) : 821-826. ScholarBank@NUS Repository.
Abstract: An ion-sensitive field effect transistor (ISFET)-based immunosensor was developed to detect/quantitate β-Bungarotoxin (β-BuTx), a potent presynaptic neurotoxin from the venom of Bungarus multicinctus. A murine monoclonal antibody (mAb 15) specific to β-BuTx was immobilized onto silicon nitride wafers after silanization and activation with glutaraldehyde. A chip based enzyme linked-immunosorbantassay (ELISA) was performed to ascertain antigen binding to the immobilized antibody. To develop an electrochemical immunosensing system for the detection/quantitation of β-BuTx, an ISFET was used as a solid phase detector. MAb 15 was immobilized on the gate region of the ISFET. The antigen antibody reaction was monitored by the addition of urease conjugated rabbit anti-β-BuTx antibodies. The sensor can detect toxin level as low as 15.6 ng/ml. The efficacy of the sensor for the determination of β-BuTx from B. multicinctus venom was demonstrated in mouse model. Toxin concentration was highest at the site of injection (748.0±26 ng/ml) and moderate amount was found in the plasma (158.5±13 ng/ml). © 2002 Elsevier Science B.V. All rights reserved.
Source Title: Biosensors and Bioelectronics
ISSN: 09565663
DOI: 10.1016/S0956-5663(02)00075-1
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Oct 8, 2019


checked on Oct 1, 2019

Page view(s)

checked on Oct 14, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.