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https://doi.org/10.1016/0006-2952(90)90488-7
Title: | Sulphate conjugation in human liver. Direct measurement of N-acetyldopamine-sulphate | Authors: | Khoo, B.Y. Sit, K.H. Wong, K.P. |
Issue Date: | 1990 | Citation: | Khoo, B.Y., Sit, K.H., Wong, K.P. (1990). Sulphate conjugation in human liver. Direct measurement of N-acetyldopamine-sulphate. Biochemical Pharmacology 40 (5) : 1021-1026. ScholarBank@NUS Repository. https://doi.org/10.1016/0006-2952(90)90488-7 | Abstract: | The overall three-step sulphate conjugating activity of the human liver was determined by the direct measurement of N-acetyldopamine (NADA)-sulphate from ATP and ionorganic sulphate NADA-sulphate was separated from NADA and quantified by HPLC-ECD (high-pressure liquid chromatography-electrochemical detection). The overall sulphate conjugation of NADA showed a pH optimum of 8.0 with apparent K(m) values for sodium sulphate and NADA of 103 μM and 4.8 μM, respectively. The optimum concentration of ATP was 5 mM and that for Mg2+ ions was 7 mM. The specific activities of 17 samples of human liver, measured by this HPLC-ECD procedure ranged from 940 to 23,128 pmol NADA-suphate/hr/mg protein. A comparison of these values with those determined by the radiometric method developed previously showed a direct correlation coefficient, r = 0.89. The rates of overall sulphate conjugation of NADA and dopamine measured in these samples by the radiometric assay procedure were also significantly correlated with r = 0.82. | Source Title: | Biochemical Pharmacology | URI: | http://scholarbank.nus.edu.sg/handle/10635/33874 | ISSN: | 00062952 | DOI: | 10.1016/0006-2952(90)90488-7 |
Appears in Collections: | Staff Publications |
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