Please use this identifier to cite or link to this item:
https://doi.org/10.1016/0006-2952(90)90488-7
DC Field | Value | |
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dc.title | Sulphate conjugation in human liver. Direct measurement of N-acetyldopamine-sulphate | |
dc.contributor.author | Khoo, B.Y. | |
dc.contributor.author | Sit, K.H. | |
dc.contributor.author | Wong, K.P. | |
dc.date.accessioned | 2012-06-08T09:30:29Z | |
dc.date.available | 2012-06-08T09:30:29Z | |
dc.date.issued | 1990 | |
dc.identifier.citation | Khoo, B.Y., Sit, K.H., Wong, K.P. (1990). Sulphate conjugation in human liver. Direct measurement of N-acetyldopamine-sulphate. Biochemical Pharmacology 40 (5) : 1021-1026. ScholarBank@NUS Repository. https://doi.org/10.1016/0006-2952(90)90488-7 | |
dc.identifier.issn | 00062952 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/33874 | |
dc.description.abstract | The overall three-step sulphate conjugating activity of the human liver was determined by the direct measurement of N-acetyldopamine (NADA)-sulphate from ATP and ionorganic sulphate NADA-sulphate was separated from NADA and quantified by HPLC-ECD (high-pressure liquid chromatography-electrochemical detection). The overall sulphate conjugation of NADA showed a pH optimum of 8.0 with apparent K(m) values for sodium sulphate and NADA of 103 μM and 4.8 μM, respectively. The optimum concentration of ATP was 5 mM and that for Mg2+ ions was 7 mM. The specific activities of 17 samples of human liver, measured by this HPLC-ECD procedure ranged from 940 to 23,128 pmol NADA-suphate/hr/mg protein. A comparison of these values with those determined by the radiometric method developed previously showed a direct correlation coefficient, r = 0.89. The rates of overall sulphate conjugation of NADA and dopamine measured in these samples by the radiometric assay procedure were also significantly correlated with r = 0.82. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0006-2952(90)90488-7 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | ANATOMY | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1016/0006-2952(90)90488-7 | |
dc.description.sourcetitle | Biochemical Pharmacology | |
dc.description.volume | 40 | |
dc.description.issue | 5 | |
dc.description.page | 1021-1026 | |
dc.description.coden | BCPCA | |
dc.identifier.isiut | A1990DZ05400015 | |
Appears in Collections: | Staff Publications |
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