Please use this identifier to cite or link to this item: https://doi.org/10.1016/0006-2952(90)90488-7
DC FieldValue
dc.titleSulphate conjugation in human liver. Direct measurement of N-acetyldopamine-sulphate
dc.contributor.authorKhoo, B.Y.
dc.contributor.authorSit, K.H.
dc.contributor.authorWong, K.P.
dc.date.accessioned2012-06-08T09:30:29Z
dc.date.available2012-06-08T09:30:29Z
dc.date.issued1990
dc.identifier.citationKhoo, B.Y., Sit, K.H., Wong, K.P. (1990). Sulphate conjugation in human liver. Direct measurement of N-acetyldopamine-sulphate. Biochemical Pharmacology 40 (5) : 1021-1026. ScholarBank@NUS Repository. https://doi.org/10.1016/0006-2952(90)90488-7
dc.identifier.issn00062952
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/33874
dc.description.abstractThe overall three-step sulphate conjugating activity of the human liver was determined by the direct measurement of N-acetyldopamine (NADA)-sulphate from ATP and ionorganic sulphate NADA-sulphate was separated from NADA and quantified by HPLC-ECD (high-pressure liquid chromatography-electrochemical detection). The overall sulphate conjugation of NADA showed a pH optimum of 8.0 with apparent K(m) values for sodium sulphate and NADA of 103 μM and 4.8 μM, respectively. The optimum concentration of ATP was 5 mM and that for Mg2+ ions was 7 mM. The specific activities of 17 samples of human liver, measured by this HPLC-ECD procedure ranged from 940 to 23,128 pmol NADA-suphate/hr/mg protein. A comparison of these values with those determined by the radiometric method developed previously showed a direct correlation coefficient, r = 0.89. The rates of overall sulphate conjugation of NADA and dopamine measured in these samples by the radiometric assay procedure were also significantly correlated with r = 0.82.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0006-2952(90)90488-7
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentANATOMY
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/0006-2952(90)90488-7
dc.description.sourcetitleBiochemical Pharmacology
dc.description.volume40
dc.description.issue5
dc.description.page1021-1026
dc.description.codenBCPCA
dc.identifier.isiutA1990DZ05400015
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