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Title: Sulphate conjugation in human liver. Direct measurement of N-acetyldopamine-sulphate
Authors: Khoo, B.Y.
Sit, K.H. 
Wong, K.P. 
Issue Date: 1990
Citation: Khoo, B.Y., Sit, K.H., Wong, K.P. (1990). Sulphate conjugation in human liver. Direct measurement of N-acetyldopamine-sulphate. Biochemical Pharmacology 40 (5) : 1021-1026. ScholarBank@NUS Repository.
Abstract: The overall three-step sulphate conjugating activity of the human liver was determined by the direct measurement of N-acetyldopamine (NADA)-sulphate from ATP and ionorganic sulphate NADA-sulphate was separated from NADA and quantified by HPLC-ECD (high-pressure liquid chromatography-electrochemical detection). The overall sulphate conjugation of NADA showed a pH optimum of 8.0 with apparent K(m) values for sodium sulphate and NADA of 103 μM and 4.8 μM, respectively. The optimum concentration of ATP was 5 mM and that for Mg2+ ions was 7 mM. The specific activities of 17 samples of human liver, measured by this HPLC-ECD procedure ranged from 940 to 23,128 pmol NADA-suphate/hr/mg protein. A comparison of these values with those determined by the radiometric method developed previously showed a direct correlation coefficient, r = 0.89. The rates of overall sulphate conjugation of NADA and dopamine measured in these samples by the radiometric assay procedure were also significantly correlated with r = 0.82.
Source Title: Biochemical Pharmacology
ISSN: 00062952
DOI: 10.1016/0006-2952(90)90488-7
Appears in Collections:Staff Publications

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