Please use this identifier to cite or link to this item: https://doi.org/10.1016/0167-4889(91)90154-P
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dc.titleSulphate induces very fast cell rounding and detachment
dc.contributor.authorSit, K.H.
dc.contributor.authorWong, K.P.
dc.date.accessioned2012-06-08T09:29:27Z
dc.date.available2012-06-08T09:29:27Z
dc.date.issued1991
dc.identifier.citationSit, K.H., Wong, K.P. (1991). Sulphate induces very fast cell rounding and detachment. Biochimica et Biophysica Acta - Molecular Cell Research 1092 (2) : 180-183. ScholarBank@NUS Repository. https://doi.org/10.1016/0167-4889(91)90154-P
dc.identifier.issn01674889
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/33808
dc.description.abstractWithin a few minutes of incubation with SO42-, cultured monolayer cells retract into round shapes with drastically reduced surface area. Concomitant elevation of phosphoinositide second messenger levels, viz, 1,2-diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), is observed. A causal relationship with sulphation seems to be suggested by finding (a) sulphation of an added acceptor, 4-methylumbelliferone, (b) sulphation of endogenous glycosaminoglycan (GAG) polymers, (c) inhibition by phenol sulphotransferase inhibitor, DCNP (2,6-dichloro-4-nitrophenol). DCNP also inhibits the second messenger production and cell rounding. Reduced surface area appears to be caused by massive plasma membrane internalization in a distinctive endocytosis which is also seen in cell rounding from directly imposed ionic gradients. Reducing the surface area would reduce the adhesive or attachment sites. Besides demonstrating a highly efficient cell detachment potential, huge macromolecules appear to be readily internalized. The association of sulphation, signal transduction and cell detachment is novel.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0167-4889(91)90154-P
dc.sourceScopus
dc.subjectcell retraction
dc.subjectendocytosis
dc.subjectsignal transduction
dc.subjectsodium ion/proton antiport
dc.subjectsulfation
dc.typeArticle
dc.contributor.departmentANATOMY
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/0167-4889(91)90154-P
dc.description.sourcetitleBiochimica et Biophysica Acta - Molecular Cell Research
dc.description.volume1092
dc.description.issue2
dc.description.page180-183
dc.description.codenBAMRD
dc.identifier.isiutA1991FK24600009
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