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Title: Beta-cyclodextrin Conjugated Magnetic Nanoparticles for Bio-and Environmental Applications
Keywords: Magnetic nanoparticles, β-cyclodextrin, protein refolding, bioseparation, organic/inorganic pollutants removal, adsortion/desortption, drug inclusion
Issue Date: 6-May-2011
Citation: ABU ZAYED MD BADRUDDOZA (2011-05-06). Beta-cyclodextrin Conjugated Magnetic Nanoparticles for Bio-and Environmental Applications. ScholarBank@NUS Repository.
Abstract: Magnetic nanoparticles (MNPs) due to their high specific surface area, biocompatibility, low toxicity and strong magnetic responsivity, have emerged as excellent materials in many fields, such as biology, medicine, environment and material science. In this work, we designed and fabricated ?-cyclodextrin (?-CD) conjugated MNPs which could be used in various bio- and environmental applications. Cyclodextrins are natural oligosaccharides which have the molecular inclusion/complexation capabilities through host-guest interactions with a wide variety of organic and inorganic molecules. Tagging cyclodextrins with magnetic, stable nanoparticles makes them magneto-responsive and may lead to a new generation of adsorbents which will provide good opportunities for applications in the fields of bio-separation/purification, contaminants removal from wastewater in environment pollution cleanup and hydrophobic drug delivery. In this thesis work, tosyl-?-cyclodextrin (Ts-?-CD) and carboxymethyl-?-cyclodextrin (CM-?-CD) conjugated Fe3O4 MNPs were fabricated using different synthetic routes. Functionalized nanoparticles were characterized with FTIR, TEM, XPS, XRD, VSM and TGA etc. The use of tosyl-?-cyclodextrin grafted 3-aminopropyltriethoxysilane (APTS) modified MNPs (CD-APTS-MNPs) as a suitable and efficient materials (stripping agent) in solid-phase artificial chaperone-assisted refolding is demonstrated using carbonic anhydrase bovine (CA) as model protein. Adsorption behaviours of carboxymethyl-?-cyclodextrin (CM-?-CD) conjugated magnetic nanocomposites with biomolecules (nucleosides), organic pollutants (methylene blue) and inorganic pollutants (Cu2+ ions) are also studied. Effects of several parameters such as pH, initial solute concentration, temperature etc. and adsorption interactions (mechanism) and desorption/regeneration of the adsorbents are also investigated and discussed. Experimental results show that CM-?-CD conjugated MNPs are promising tools for the bio-separation/purification and organic/inorganic pollutants removal from aqueous solution in environment pollution cleanup. In addition, the study on biomolecules separation provides a promising way to separate organic molecules based on the difference of binding property by forming host?guest inclusion. Highly uniform core-shell magnetic nanocomposite materials [Fe3O4@SiO2(FITC)-FA/CMCD NPs] possessing an assortment of functionalities: superparamagnetism, luminescence, cell-targeting, hydrophobic drug storage and delivery are also fabricated in this work. Our preliminary results suggest that such core-shell nanocomposite can be a smart theranostic candidate for simultaneous bioimaging, magnetic control, cancer cell-targeting and drug delivery.
Appears in Collections:Ph.D Theses (Open)

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