Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ejca.2009.06.021
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dc.titlePositive association between nuclear Runx2 and oestrogen-progesterone receptor gene expression characterises a biological subtype of breast cancer
dc.contributor.authorDas, K.
dc.contributor.authorPutti, T.
dc.contributor.authorSalto-Tellez, M.
dc.contributor.authorLeong, D.T.
dc.contributor.authorGupta, A.
dc.contributor.authorvan, Wijnen A.J.
dc.contributor.authorShen, L.
dc.contributor.authorStein, G.S.
dc.date.accessioned2012-05-29T02:21:39Z
dc.date.available2012-05-29T02:21:39Z
dc.date.issued2009
dc.identifier.citationDas, K., Putti, T., Salto-Tellez, M., Leong, D.T., Gupta, A., van, Wijnen A.J., Shen, L., Stein, G.S. (2009). Positive association between nuclear Runx2 and oestrogen-progesterone receptor gene expression characterises a biological subtype of breast cancer. European Journal of Cancer 45 (13) : 2239-2248. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ejca.2009.06.021
dc.identifier.issn09598049
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/33179
dc.description.abstractPurpose: The runt-related transcription factor, Runx2 may have an oncogenic role in mediating metastatic events in breast cancer, but whether Runx2 has a role in the early phases of breast cancer development is not clear. We examined the expression of Runx2 and its relationship with oestrogen receptor (ER) and progesterone receptor (PR) in breast cancer cell lines and tissues. Methods: Two human breast cancer cell lines, MCF-7 and MDA-MB-231 were transiently transfected with vectors expressing either Runx2 or ER and the levels of both proteins and mRNA were examined by Western blot analysis and quantitative real-time PCR, respectively. Runx2 expression was also examined in tissue microarray sections of 123 breast cancer patients by immunohistochemistry and results were correlated with clinico-pathological characteristics. Results: Expression of Runx2 and ER was reciprocal in the breast cell culture models and Runx2 suppressed ERβ but not ERα mRNA levels. In contrast, functional expression of Runx2 was evident in the nucleus in 28% of the breast cancer tissues and in both early and late stages of tumour growth. Importantly, Runx2 expression was significantly more frequent in Grade 2 compared to Grade 1 and Grade 3 tumours (48% versus 39% versus 13%) and the expression was significantly associated with ER (p = 0.005), PR (p = 0.008) expressions in Grade 2 & Grade 3 tumours than Grade 1 tumours. Conclusion: We propose that Runx2, ER and PR triple positivity in Grades 2 and 3 defines a biological subtype in breast cancer. © 2009 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ejca.2009.06.021
dc.sourceScopus
dc.subjectBreast cancer
dc.subjectCerbB2
dc.subjectOestrogen receptor
dc.subjectProgesterone receptor
dc.subjectRunx2
dc.typeArticle
dc.contributor.departmentPATHOLOGY
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.contributor.departmentNATIONAL UNIVERSITY MEDICAL INSTITUTES
dc.description.doi10.1016/j.ejca.2009.06.021
dc.description.sourcetitleEuropean Journal of Cancer
dc.description.volume45
dc.description.issue13
dc.description.page2239-2248
dc.description.codenEJCAE
dc.identifier.isiut000270645700010
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