Please use this identifier to cite or link to this item:
|Title:||TRANSCRIPTOMIC ANALYSIS OF MAMMALIAN SPINAL NEURULATION||Authors:||CAO SHOUFENG||Keywords:||Laser capture microdissection,Mammalian spinal neurulation,Transcriptomic analysis,High-density oligonucleotide microarray, β-1, 4-galactosyltransfera||Issue Date:||18-Aug-2011||Citation:||CAO SHOUFENG (2011-08-18). TRANSCRIPTOMIC ANALYSIS OF MAMMALIAN SPINAL NEURULATION. ScholarBank@NUS Repository.||Abstract:||Neural tube closure, a process in neurulation, is critical for formation of the central nervous system. Failure of neural tube closure leads to neural tube defects. To identify the molecular regulation of closure, transcriptomic profiling of neurulation was performed using laser capture microdissected neuroepithelium and the surrounding mesenchyme in combination with Affymetrix microarray technology. Various candidate genes involved in regulating median and dorsolateral hinge point formation were identified and groped by signaling pathways and functions based on their Gene Ontology classification. Numerous glycosaminoglycan-related genes, including ß-1, 4-galactosyltransferase which codes for an enzyme involved in initiation of glycosaminoglycan biosynthesis, were found to be differentially expressed in the profiles. The function of this enzyme was investigated using siRNA to silence its expression in NE4C neuroepithelial cells.||URI:||http://scholarbank.nus.edu.sg/handle/10635/33043|
|Appears in Collections:||Ph.D Theses (Open)|
Show full item record
Files in This Item:
|Cao SF-PDF.pdf||2.61 MB||Adobe PDF|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.