Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0006-291X(03)00612-0
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dc.titleIL-10 synergistically enhances GM-CSF-induced CCR1 expression in myelomonocytic cells
dc.contributor.authorLi, H.
dc.contributor.authorChoo, H.H.
dc.contributor.authorChan, J.H.P.
dc.contributor.authorFred, Wong W.S.
dc.contributor.authorCheung, W.
dc.contributor.authorLai, P.S.
dc.date.accessioned2012-04-02T07:55:09Z
dc.date.available2012-04-02T07:55:09Z
dc.date.issued2003
dc.identifier.citationLi, H., Choo, H.H., Chan, J.H.P., Fred, Wong W.S., Cheung, W., Lai, P.S. (2003). IL-10 synergistically enhances GM-CSF-induced CCR1 expression in myelomonocytic cells. Biochemical and Biophysical Research Communications 304 (2) : 417-424. ScholarBank@NUS Repository. https://doi.org/10.1016/S0006-291X(03)00612-0
dc.identifier.issn0006291X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/32274
dc.description.abstractCC chemokine receptor 1 (CCR1) has been implicated in inflammation. The present study examined the signaling mechanisms that mediate GM-CSF/IL-10-induced synergistic CCR1 protein expression in monocytic U937 cells. GM-CSF alone markedly increased both the mRNA and protein expression of CCR1. IL-10 augmented GM-CSF-induced CCR1 protein expression with no effect on mRNA expression. PD098059 and U0126 (two MEK inhibitors), and LY294002 (a PI3K inhibitor) inhibited GM-CSF/IL-10-induced CCR1 gene and protein expression. PD098059, U0126, and LY294002 also attenuated chemotaxis of GM-CSF/IL-10-primed U937 cells in response to MIP-1α. Immunoblotting studies show that GM-CSF alone induced ERK2 phosphorylation; whereas, IL-10 alone induced p70S6k phosphorylation in U937 cells. Neither cytokine when used alone induced PKB/Akt phosphorylation. Combined GM-CSF/IL-10 treatment of U937 cells induced phosphorylation of ERK2, p70S6k, and PKB/Akt. PD098059 and U0126 completely abrogated ERK2 phosphorylation; whereas, LY294002 completely blocked PKB/Akt and p70S6k phosphorylation. Our findings indicate that IL-10 may potentiate GM-CSF-induced CCR1 protein expression in U937 cells via activation of PKB/Akt and p70S6k. © 2003 Elsevier Science (USA). All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0006-291X(03)00612-0
dc.sourceScopus
dc.subjectCCR1
dc.subjectGM-CSF
dc.subjectIL-10
dc.subjectLY294002
dc.subjectMIP-1α
dc.subjectp70S6k
dc.subjectPD098059
dc.subjectPKB/Akt
dc.subjectU0126
dc.subjectU937
dc.typeArticle
dc.contributor.departmentPHARMACOLOGY
dc.contributor.departmentPAEDIATRICS
dc.description.doi10.1016/S0006-291X(03)00612-0
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume304
dc.description.issue2
dc.description.page417-424
dc.description.codenBBRCA
dc.identifier.isiut000182809900032
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