Please use this identifier to cite or link to this item:
https://doi.org/10.1016/0166-0934(94)00104-O
DC Field | Value | |
---|---|---|
dc.title | Rapid, single-step RT-PCR typing of dengue viruses using five NS3 gene primers | |
dc.contributor.author | Seah, C.L.K. | |
dc.contributor.author | Chow, V.T.K. | |
dc.contributor.author | Tan, H.C. | |
dc.contributor.author | Chan, Y.C. | |
dc.date.accessioned | 2012-03-28T05:50:28Z | |
dc.date.available | 2012-03-28T05:50:28Z | |
dc.date.issued | 1995 | |
dc.identifier.citation | Seah, C.L.K., Chow, V.T.K., Tan, H.C., Chan, Y.C. (1995). Rapid, single-step RT-PCR typing of dengue viruses using five NS3 gene primers. Journal of Virological Methods 51 (2-3) : 193-200. ScholarBank@NUS Repository. https://doi.org/10.1016/0166-0934(94)00104-O | |
dc.identifier.issn | 01660934 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/31305 | |
dc.description.abstract | In order to detect and type dengue viruses in serum specimens, four type-specific downstream primers were designed for use with a consensus upstream primer in a reverse transcription and polymerase chain reaction (RT-PCR) assay. RT-PCR using these five primers amplified NS3 gene fragments of diagnostic sizes of 169, 362, 265 and 426 base pairs for dengue virus types 1, 2, 3 and 4, respectively, but not for Japanese encephalitis, Kunjin and yellow fever viruses. The conventional two-step RT-PCR procedure was simplified by combining RT and PCR in a single-step format with a 'hot start'. This RT-PCR protocol was applied successfully to dengue virus-spiked serum and dengue patient serum samples, and could detect as few as one PFU of dengue virus. This assay offers a rapid, specific and sensitive molecular technique for the simultaneous detection and typing of dengue viruses. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0166-0934(94)00104-O | |
dc.source | Scopus | |
dc.subject | dengue virus | |
dc.subject | NS3 gene | |
dc.subject | single-step RT-PCR | |
dc.subject | typing | |
dc.type | Article | |
dc.contributor.department | MICROBIOLOGY | |
dc.description.doi | 10.1016/0166-0934(94)00104-O | |
dc.description.sourcetitle | Journal of Virological Methods | |
dc.description.volume | 51 | |
dc.description.issue | 2-3 | |
dc.description.page | 193-200 | |
dc.description.coden | JVMED | |
dc.identifier.isiut | A1995QG92600006 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.