Please use this identifier to cite or link to this item:
|Title:||Localization of linear cytotoxic and pro-apoptotic epitopes in RTX toxin ApxIII of Actinobacillus pleuropneumoniae||Authors:||Seah, J.N.
|Issue Date:||2004||Citation:||Seah, J.N., Kwang, J. (2004). Localization of linear cytotoxic and pro-apoptotic epitopes in RTX toxin ApxIII of Actinobacillus pleuropneumoniae. Vaccine 22 (11-12) : 1494-1497. ScholarBank@NUS Repository. https://doi.org/10.1016/j.vaccine.2003.10.017||Abstract:||RTX toxin ApxIII secreted by Actinobacillus pleuropneumoniae affects porcine pulmonary alveolar macrophages and neutrophils. The distribution of ApxIII linear cytotixic and pro-apoptotic determinants was characterized by neutrophil protection assay, DNA fragmentation and caspase-3 activity using antisera produced against its N-terminus, hydrophobic domain, activation domain, calcium-binding domain and C-terminal half. Neutralization of ApxIII cytotoxicity and pro-apoptotic acitivities was found in antisera raised against its N- and C-termini whereas ApxIII activation domain-specific antiserum expressed cytotoxic-neutralizing activity. No neutralizing activities were detected in antisera against other structural domains of ApxIII suggesting that the putative linear cytotoxic and pro-apoptotic determinants of ApxIII were mapped to its terminal domains and activation domains. © 2003 Elsevier Ltd. All rights reserved.||Source Title:||Vaccine||URI:||http://scholarbank.nus.edu.sg/handle/10635/31288||ISSN:||0264410X||DOI:||10.1016/j.vaccine.2003.10.017|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 21, 2019
WEB OF SCIENCETM
checked on May 14, 2019
checked on May 12, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.