Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biochi.2005.03.003
DC FieldValue
dc.titleProgrammed cell death in Blastocystis hominis occurs independently of caspase and mitochondrial pathways
dc.contributor.authorNasirudeen, A.M.A.
dc.contributor.authorTan, K.S.W.
dc.date.accessioned2012-03-28T05:49:47Z
dc.date.available2012-03-28T05:49:47Z
dc.date.issued2005
dc.identifier.citationNasirudeen, A.M.A., Tan, K.S.W. (2005). Programmed cell death in Blastocystis hominis occurs independently of caspase and mitochondrial pathways. Biochimie 87 (6) : 489-497. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biochi.2005.03.003
dc.identifier.issn03009084
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/31269
dc.description.abstractWe demonstrated previously that a cytotoxic monoclonal antibody (MAb) 1D5 elicits a programmed cell death (PCD) response in Blastocystis hominis and showed that caspase-3-like protease influences but is not essential for PCD in MAb 1D5-treated B. hominis. We also showed that mitochondrial dysregulation played a role in cell death. In the current study, we further analyzed the signaling pathways involved in PCD mediated by MAb 1D5. B. hominis cells were treated with MAb 1D5 or control MAb 5, either with or without pretreatment with a pan-caspase inhibitor, zVAD.fmk, and/or a mitochondrial transition pore blocker, cyclosporine A (CA). Flow cytometric examination of cell size, mitochondrial membrane potential (ΔΨm), caspase activation and in situ DNA fragmentation showed that zVAD.fmk and CA, used independently or in combination, failed to inhibit MAb 1D5-mediated PCD. Interestingly, cell exposure to either inhibitor resulted in partial inhibition of DNA fragmentation while combined exposure of cells to inhibitors abolished DNA fragmentation completely. This study sheds new light on the conserved nature of PCD pathways in parasitic protozoa and is also the first report describing caspase- and mitochondria-independent cell death pathways in a protozoan parasite. © 2005 Elsevier SAS. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biochi.2005.03.003
dc.sourceScopus
dc.subjectBlastocystis
dc.subjectCaspases
dc.subjectCyclosporin A
dc.subjectProgrammed cell death
dc.subjectzVAD.fmk
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.biochi.2005.03.003
dc.description.sourcetitleBiochimie
dc.description.volume87
dc.description.issue6
dc.description.page489-497
dc.description.codenBICMB
dc.identifier.isiut000229895700001
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