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Title: | In vitro conversion of penicillin G and ampicillin by recombinant Streptomyces clavuligerus NRRL 3585 deacetoxycephalosporin C synthase | Authors: | Sim, J. Sim, T.-S. |
Keywords: | Ampicillin Ascorbate Deacetoxycephalosproin C synthase Dithiothreitol Penicillin G Ring expansion |
Issue Date: | 2001 | Citation: | Sim, J., Sim, T.-S. (2001). In vitro conversion of penicillin G and ampicillin by recombinant Streptomyces clavuligerus NRRL 3585 deacetoxycephalosporin C synthase. Enzyme and Microbial Technology 29 (4-5) : 240-245. ScholarBank@NUS Repository. | Abstract: | The in vitro conversion of penicillin N analogues to cephalosporins was first demonstrated in resting cells and cell-free extracts of Streptomyces clavuligerus NP1. This study has optimized the conversion rate of a highly expressed soluble source of recombinant S. clavuligerus NRRL 3585 deacetoxycepalosporin C synthase (scDAOCS) through detailed characterization of its cofactor requirements. Inclusion of dithiothreitol (DTT) and ascorbate at various concentrations from 0.2-16 mM and 0.2-10 mM was found to be inhibitory to scDAOCS activity under specified reaction conditions examined. Simultaneous omission of these cofactors in a new modified reaction condition, comprising of 50 mM Tris HCl (pH7.4), 0.8 mM ATP, 1.28 mM α-ketoglutarate and 1.8 mM FeSO4, enhanced the conversion rate of two penicillin N analogues, penicillin G and ampicillin, through 20- and 35-fold respectively. © 2001 Elsevier Science Inc. All rights reserved. | Source Title: | Enzyme and Microbial Technology | URI: | http://scholarbank.nus.edu.sg/handle/10635/31108 | ISSN: | 01410229 |
Appears in Collections: | Staff Publications |
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