Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-1097(97)00085-2
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dc.titleCharacterization of IS1474, an insertion sequence of the IS21 family isolated from Pseudomonas alcaligenes NCIB 9867
dc.contributor.authorYeo, C.C.
dc.contributor.authorPoh, C.L.
dc.date.accessioned2012-03-28T05:46:35Z
dc.date.available2012-03-28T05:46:35Z
dc.date.issued1997
dc.identifier.citationYeo, C.C., Poh, C.L. (1997). Characterization of IS1474, an insertion sequence of the IS21 family isolated from Pseudomonas alcaligenes NCIB 9867. FEMS Microbiology Letters 149 (2) : 257-263. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1097(97)00085-2
dc.identifier.issn03781097
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/31099
dc.description.abstractA new insertion sequence (IS) designated IS1474 was isolated from Pseudomonas alcaligenes NCIB 9867 (P25X). IS1474 is a 2632 bp element which showed a characteristic IS structure with 12 bp inverted repeats (IRs) flanking a 2608 bp central region. IS1474 contained four open reading frames (ORF1-ORF4), two in each orientation. Similarities were detected between ORF1 and ORF2 and the putative transposases of the IS21 family. Sequences upstream from IS1474 were found to display up to 89% homology with IS53 from Pseudomonas syringae suggesting that IS1474 had inserted into another related IS element designated IS1475. An open reading frame, ORF5, located at the junction of IS1474 and IS1475, showed similarities with the IstB protein of IS21 and could possibly be the transposase subunit of IS1475. Transposition assays showed that IS1474 transposed at a relatively low frequency leading to cointegration with target plasmids. Hybridization studies showed that IS1474 is present in at least 13 copies in the chromosome of P25X and one copy on its endogenous plasmid.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0378-1097(97)00085-2
dc.sourceScopus
dc.subjectBacterial insertion sequence
dc.subjectIS1474
dc.subjectPseudomonas alcaligenes
dc.subjectTransposase
dc.subjectTransposition
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/S0378-1097(97)00085-2
dc.description.sourcetitleFEMS Microbiology Letters
dc.description.volume149
dc.description.issue2
dc.description.page257-263
dc.description.codenFMLED
dc.identifier.isiutA1997WV19500018
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