Please use this identifier to cite or link to this item:
Title: Liver Fibrosis Surface Assessment Based on Non-Linear Optical Microscopy
Authors: HE YUTING
Keywords: liver fibrosis, microscopy, SHG, TPEF, imaging
Issue Date: 5-Aug-2010
Citation: HE YUTING (2010-08-05). Liver Fibrosis Surface Assessment Based on Non-Linear Optical Microscopy. ScholarBank@NUS Repository.
Abstract: Monitoring liver fibrosis progression by liver biopsy is important for certain treatment decisions but repeated biopsy is invasive. We envision redefinition or elimination of liver biopsy with surface scanning of the liver with minimally invasive optical methods. To achieve this, we acquired second harmonic generation and two-photon excitation fluorescence microscopy images from fibrotic liver tissues. We established a quantification methods for fibrosis as-sessment based on morphological features extraction and analysis of such im-ages. Using this quantification method, we further defined capsule and sub-capsule regions on the liver surface based on image analysis of features. We discovered a strong correlation between the liver fibrosis progression on the surface and liver interior where biopsy is typically obtained. With the infor-mation contained on liver surfaces reflecting the fibrosis progression in liver interior, a window based animal model was later developed for live imaging and assessment of fibrosis through liver surface scanning.
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
HeYT.pdf6.6 MBAdobe PDF



Page view(s)

checked on Apr 19, 2019


checked on Apr 19, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.