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Title: Rapid Construction of Mechanically-Confined Multi-Cellular Structures Using Dendrimeric Inter-Cellular Linker
Authors: MO XUEJUN
Keywords: Rapid, dendrimer, tissue engineering, oleyl-PEG, multi- cellular structures, defined cellular constructs
Issue Date: 25-May-2011
Citation: MO XUEJUN (2011-05-25). Rapid Construction of Mechanically-Confined Multi-Cellular Structures Using Dendrimeric Inter-Cellular Linker. ScholarBank@NUS Repository.
Abstract: Tissue constructs that mimic the in vivo cell- cell and cell- matrix interactions are especially useful for applications involving the cell- dense and matrix- poor internal organs. Rapid and precise arrangement of cells into functional tissue constructs remains a challenge in tissue engineering. We have developed a dendrimeric inter- cellular linker that can rapidly and effectively induce formation of multi- cellular structures, through the cross- linking of live cells via anchorage of hydrophobic oleyl groups at the end terminal of the dendrimeric linker into the cell membrane surface. We demonstrate rapid assembly of C3A cells into multi- cell structures using the dendrimeric inter- cellular linker. Bringing linker- treated cells into close proximity to each other via mechanical means such as centrifugation and micromanipulation enables their rapid assembly into multi- cellular structures within minutes. The multi- cellular structures exhibit high levels of viability, proliferation, three- dimensional (3D) cell morphology and improved cellular functions over a 7- day culture period. The linker stabilizes the multi- cellular structures of defined shape and pattern in a gel- free environment by mechanically confining the cells. Defined multi- cellular structures such as rings, sheets or branching rods can be built that can serve as potential tissue building blocks to be further assembled into complex 3D tissue constructs for artificial organ construction and other biomedical applications e.g. in vitro models for human disease study.
Appears in Collections:Ph.D Theses (Open)

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