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|Title:||CHARACTERIZATION OF INTERACTIONS BETWEEN HEPATITIS C VIRUS AND HOST PROTEINS AND THEIR EFFECTS ON APOPTOSIS||Authors:||NUR KHAIRIAH BINTE MOHD ISMAIL||Keywords:||HCV, apoptosis, core, Bcl-2 protein family||Issue Date:||29-Sep-2011||Citation:||NUR KHAIRIAH BINTE MOHD ISMAIL (2011-09-29). CHARACTERIZATION OF INTERACTIONS BETWEEN HEPATITIS C VIRUS AND HOST PROTEINS AND THEIR EFFECTS ON APOPTOSIS. ScholarBank@NUS Repository.||Abstract:||Hepatitis C virus (HCV) is a leading cause of chronic liver disease. Comprehensive understanding of HCV replication and pathogenesis is crucial for the development of successful therapies. The aim of this project is to identify and characterize interactions between HCV and host proteins, specifically in relation to apoptosis. We have identified a Bcl-2 homology 3 (BH3) domain in the HCV core protein that is essential for its proapoptotic property and for specific interaction with the human myeloid cell factor 1 (Mcl-1), a prosurvival member of the Bcl-2 family. Elevated levels of endogenous Bcl-XL, another prosurvival Bcl-2 protein, were detected in HCV-infected Huh7.5 cells. Suppression of Bcl-XL upregulation in HCV-infected cells by small interfering RNA (siRNA) significantly increased apoptosis, suggesting a role for Bcl-XL in modulating HCV-mediated apoptosis. Gene expression profiling of HCV-infected cells by quantitative PCR array analysis was also performed to identify host apoptotic genes that are transcriptionally regulated by infection.||URI:||http://scholarbank.nus.edu.sg/handle/10635/30686|
|Appears in Collections:||Ph.D Theses (Open)|
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