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Title: Platelet-endothelial cell adhesion molecule-1 gene polymorphism and its soluble level are associated with severe coronary artery stenosis in Chinese Singaporean
Authors: Wei, H.
Fang, L.
Chowdhury, S.H.
Gong, N.
Song, J.
Wu, S.
Lim, Y.L.
Chatterjee, S. 
Xiong, Z.
Mak, K.H.
Koay, E. 
Sethi, S. 
Keywords: Atherosclerosis
Coronary artery disease
Gene polymorphism
Issue Date: 2004
Citation: Wei, H., Fang, L., Chowdhury, S.H., Gong, N., Song, J., Wu, S., Lim, Y.L., Chatterjee, S., Xiong, Z., Mak, K.H., Koay, E., Sethi, S. (2004). Platelet-endothelial cell adhesion molecule-1 gene polymorphism and its soluble level are associated with severe coronary artery stenosis in Chinese Singaporean. Clinical Biochemistry 37 (12) : 1091-1097. ScholarBank@NUS Repository.
Abstract: Platelet-endothelial cell adhesion molecule-1 (PECAM-1) mediates the transendothelial migration of circulating leukocytes, a characteristic change in vascular inflammation leading to atherosclerotic plaque development. We hypothesized that genetic variation and soluble level of PECAM-1 could be associated with coronary artery disease (CAD). We analyzed two single nucleotide polymorphisms (SNPs) of PECAM-1 gene C+373G (Leu125Val) at exon 3, which encodes the first extracellular (Ig)-like domain that mediates the homophilic binding of PECAM-1, and G+1688A (Ser563Asn) at exon 8 in 144 angiographically documented (≥70% stenosis) patients with CAD and 150 age- and sex-matched controls in the Chinese population in Singapore, using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) strategy. Level of plasma soluble PECAM-1 (sPECAM-1) was measured by ELISA. The Leu125Val polymorphism was associated with CAD (P < 0.01). Also, the level of sPECAM-1 is was found to be elevated in CAD patients (P = 0.005). Moreover, subjects with the homozygous GG genotype of the Leu125Val polymorphism had higher sPECAM-1 levels (P = 0.005). The level of sPECAM-1 was further correlated to soluble platelet selectin (sP-selectin, also measured by ELISA), platelet count, and total white blood cell count (WBC), suggesting that platelets are a major source of sPECAM-1 and platelet activation and inflammation may contribute to PECAM-1 elevations in CAD patients. The Leu125Val polymorphism of PECAM-1 and the level of sPECAM-1 are associated with CAD in Chinese in Singapore. The level of sPECAM-1 is also associated with platelet activation and inflammation and correlated to the Leu125Val polymorphism. Our data suggest that PECAM-1 plays an important role in the development of atherosclerosis. © 2004 The Canadian Society of Clinical Chemists. All rights reserved.
Source Title: Clinical Biochemistry
ISSN: 00099120
DOI: 10.1016/j.clinbiochem.2004.08.006
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