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|Title:||Intrinsic variability in the detection of micrometastases in lymph nodes for re-staging of colorectal cancer: Effect of individual markers and tissue samples||Authors:||Salto-Tellez, M.
|Issue Date:||2003||Citation:||Salto-Tellez, M., Kong, S.L., Koay, E.S.C., Leong, A.P.K. (2003). Intrinsic variability in the detection of micrometastases in lymph nodes for re-staging of colorectal cancer: Effect of individual markers and tissue samples. European Journal of Cancer 39 (9) : 1234-1241. ScholarBank@NUS Repository. https://doi.org/10.1016/S0959-8049(03)00231-4||Abstract:||In this study, we investigated whether (a) carcinoembryonic antigen (CEA), cytokeratin-20 (CK-20) and guanylyl cyclase C (GCC) are clinically useful markers for the molecular detection of submicroscopic metastases in colorectal cancer (CRC) and (b) whether overexpression of CEA, CK-20 and GCC can be reliably detected in formalin-fixed, paraffin-embedded tissues as well as frozen lymph nodes. We studied 175 frozen lymph nodes and 158 formalin-fixed, paraffin-embedded lymph nodes from 28 cases of CRC. CEA or CK-20 or GCC-specific polymerase chain reaction (PCR) was carried out on mRNA transcripts extracted from the nodal tissues. Ten out of 11 Dukes' B CRC cases had detectable CEA and CK-20 while 6 out of 11 Dukes' B CRC cases had detectable GCC. In general, the difference of re-staged cases when comparing frozen and paraffin-embedded samples was marked; the only statistically significant correlation between frozen and paraffin tissue was for the CEA marker. Our results indicated a high incidence (>50%) of detecting micrometastases in histologically-negative lymph nodes at the molecular level. © 2003 Elsevier Science Ltd. All rights reserved.||Source Title:||European Journal of Cancer||URI:||http://scholarbank.nus.edu.sg/handle/10635/30414||ISSN:||09598049||DOI:||10.1016/S0959-8049(03)00231-4|
|Appears in Collections:||Staff Publications|
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