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Title: Understanding the functional roles of Intrinsic Protein disorder in NFkB Transcription factors
Keywords: NFkB, Transcription Factors, Disorder, Database, Dynamics, Signaling
Issue Date: 28-Dec-2011
Citation: LIM SHEN JEAN (2011-12-28). Understanding the functional roles of Intrinsic Protein disorder in NFkB Transcription factors. ScholarBank@NUS Repository.
Abstract: Protein dynamics, particularly, intrinsic protein disorder has been implicated in cellular functions. Intrinsic protein disorder contributes to transcription and cell signalling through the accommodation of multiple interaction partners and modification sites, and provision of regulation flexibility. Here, in support with previous studies, I hypothesize that analogous with sequence conservation of functionally important sites, intrinsic protein disorder properties are evolutionary conserved. To further support and test this hypothesis, in the more specific context of transcriptional regulation in cell signaling, I developed an in silico analysis pipeline for the identification of intrinsically disordered protein residues, data mining and indepth analysis of the conservation, localization and function of predicted disordered regions. The Nuclear Factor Kappa-light-chain-enhancer of Activated B cells (NFkB/Rel), important for a variety of processes including cell survival, inflammation and immunity, was chosen as the exemplar protein for this study. The findings highlight distinctive key roles of conserved disordered and nondisordered in different aspects of NFkB function. Differences in the distribution and conservation patterns of protein disorder in each NFkB protein type raise the possibility of conserved disorder signatures in different protein families, which, if true, will prove valuable for functional characterization. On a larger scale, this project shows a meaningful perspective for the understanding of protein function, through intrinsic protein disorder. The analysis pipeline developed in this study will be instrumental for large-scale functional studies of protein families. Findings from this project will also contribute to scientific knowledge in transcriptional regulation and cell signaling.
Appears in Collections:Master's Theses (Open)

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