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Title: Quality control in traditional Chinese medicines: An NMR fingerprinting approach
Keywords: Herbal Medicines, NMR, Fingerprinting, Authentication, Adulteration
Issue Date: 23-Aug-2010
Citation: LAU HIU FUNG (2010-08-23). Quality control in traditional Chinese medicines: An NMR fingerprinting approach. ScholarBank@NUS Repository.
Abstract: This thesis covers various aspects of quality control of tradition Chinese herbal medicines with NMR fingerprinting as the analytical platform. In Chapter One,a brief comparison between the holistic approach of traditional Chinese medicines (TCMs) and the reductive mechanism of Western drug is given to illustrate the problems associated with the application of the conventional quality control methods on herbal plant. Selected examples on various fingerprinting techniques are given to demonstrate the versatilities and advantages of this strategy over the `marker-approach¿ of quality measurements. Chapter Two describes the use of NMR fingerprints in the identification of unknown herbal samples. Since no chromatographic or electrophoretic separation is required, a high throughput screening can be achieved with the spectroscopic fingerprinting. A databank can be established based on authentic medical plants and this library can be subsequently used as a benchmark for similarity comparison and classifications. Various discriminant analysis models were tested and optimized to allow maximum characteristic features to be included to allow botanical identity recognition. Chapter Three deals with the analysis of herbal mixtures. Through establishing the correlation between certain unique pattern in the spectral fingerprints and the herbal composition in a mixture with multivariate models, authentication and relative quantification of unknown mixtures can be achieved with a pre-acquired fingerprint library. This strategy is useful for both the detection of herbal adulterants and the quality control of TCM formula. In Chapter Four, the application of NMR fingerprinting-chemometrics strategy is further extended to the detection of small foreign substances. This study attempts to provide a solution addressing the concerns of adulteration of herbal medicines with orthodox drugs or contamination with agrochemical residues. Multivariate models were constructed based on series of `contaminated¿ samples so that the feasibility of differentiating structurally similar foreign substances and detecting very small bioactive molecules could be demonstrated.
Appears in Collections:Ph.D Theses (Open)

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