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https://scholarbank.nus.edu.sg/handle/10635/30269
DC Field | Value | |
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dc.title | Effects of Hydrogen Peroxide on Different Models of Wound Healing | |
dc.contributor.author | LOO ENG KIAT ALVIN | |
dc.date.accessioned | 2012-01-31T18:00:50Z | |
dc.date.available | 2012-01-31T18:00:50Z | |
dc.date.issued | 2011-08-17 | |
dc.identifier.citation | LOO ENG KIAT ALVIN (2011-08-17). Effects of Hydrogen Peroxide on Different Models of Wound Healing. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/30269 | |
dc.description.abstract | It has been established that low concentrations of H<sub>2</sub>O<sub>2</sub> are produced in wounds. Yet at the same time, there is evidence that excessive oxidative damage is correlated with chronic wounds. In this thesis we explored the effects of H<sub>2</sub>O<sub>2</sub> in keratinocyte cell culture models and an <i>in vivo</i> excision wound model of wound healing. H<sub>2</sub>O<sub>2</sub> stimulates a persistent ERK phosphorylation in HaCaT keratinocytes which was found to be important in cell proliferation and migration. H<sub>2</sub>O<sub>2</sub> also increases the production of proinflammatory and pro-angiogenic cytokines such as Vascular endothelial growth factor, Interleukin-8, Granulocyte-macrophage colony-stimulating factor, Tumor necrosis factor-a, interleukin-6 and Interferon gamma-induced protein 10, in HaCaT keratinocytes. H<sub>2</sub>O<sub>2</sub> was found to increase re-epithelialization in a primary fibroblast-keratinocyte co-culture model as well. In a C57BL/6 mice excision wound model, low concentrations of H<sub>2</sub>O<sub>2</sub> (10 mM) were found to enhance angiogenesis while high concentrations of H<sub>2</sub>O<sub>2</sub> (166 mM) retarded wound closure and connective tissue formation. High concentrations of H<sub>2</sub>O<sub>2</sub> also increased the levels of MMP-8 in the wounds, which could be the cause of reduced connective tissue formation. Wounding was found to increase oxidative lipid damage, as measured by F<sub>2</sub>-isoprostanes, but H<sub>2</sub>O<sub>2</sub> treatment does not significantly increase it even at concentrations that delay wound healing. This challenges the putative claim that oxidative damage contributes to the pathology of poor healing wounds. | |
dc.language.iso | en | |
dc.subject | Wound healing, hydrogen peroxide, H2O2, ROS, oxidative damage, MAPK | |
dc.type | Thesis | |
dc.contributor.department | NUS GRAD SCH FOR INTEGRATIVE SCI & ENGG | |
dc.contributor.supervisor | HALLIWELL, BARRY | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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LooEKA.pdf | 2.55 MB | Adobe PDF | OPEN | None | View/Download |
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