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|Title:||Effects of Hydrogen Peroxide on Different Models of Wound Healing||Authors:||LOO ENG KIAT ALVIN||Keywords:||Wound healing, hydrogen peroxide, H2O2, ROS, oxidative damage, MAPK||Issue Date:||17-Aug-2011||Citation:||LOO ENG KIAT ALVIN (2011-08-17). Effects of Hydrogen Peroxide on Different Models of Wound Healing. ScholarBank@NUS Repository.||Abstract:||It has been established that low concentrations of H2O2 are produced in wounds. Yet at the same time, there is evidence that excessive oxidative damage is correlated with chronic wounds. In this thesis we explored the effects of H2O2 in keratinocyte cell culture models and an in vivo excision wound model of wound healing. H2O2 stimulates a persistent ERK phosphorylation in HaCaT keratinocytes which was found to be important in cell proliferation and migration. H2O2 also increases the production of proinflammatory and pro-angiogenic cytokines such as Vascular endothelial growth factor, Interleukin-8, Granulocyte-macrophage colony-stimulating factor, Tumor necrosis factor-a, interleukin-6 and Interferon gamma-induced protein 10, in HaCaT keratinocytes. H2O2 was found to increase re-epithelialization in a primary fibroblast-keratinocyte co-culture model as well. In a C57BL/6 mice excision wound model, low concentrations of H2O2 (10 mM) were found to enhance angiogenesis while high concentrations of H2O2 (166 mM) retarded wound closure and connective tissue formation. High concentrations of H2O2 also increased the levels of MMP-8 in the wounds, which could be the cause of reduced connective tissue formation. Wounding was found to increase oxidative lipid damage, as measured by F2-isoprostanes, but H2O2 treatment does not significantly increase it even at concentrations that delay wound healing. This challenges the putative claim that oxidative damage contributes to the pathology of poor healing wounds.||URI:||http://scholarbank.nus.edu.sg/handle/10635/30269|
|Appears in Collections:||Ph.D Theses (Open)|
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