Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2009.05.062
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dc.titleTracking transplanted cells in live animal using upconversion fluorescent nanoparticles
dc.contributor.authorIdris, N.M.
dc.contributor.authorLi, Z.
dc.contributor.authorZhang, Y.
dc.contributor.authorYe, L.
dc.contributor.authorWei, Sim E.K.
dc.contributor.authorMahendran, R.
dc.contributor.authorHo, P.C.-L.
dc.date.accessioned2012-01-30T09:45:30Z
dc.date.available2012-01-30T09:45:30Z
dc.date.issued2009
dc.identifier.citationIdris, N.M., Li, Z., Zhang, Y., Ye, L., Wei, Sim E.K., Mahendran, R., Ho, P.C.-L. (2009). Tracking transplanted cells in live animal using upconversion fluorescent nanoparticles. Biomaterials 30 (28) : 5104-5113. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2009.05.062
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/30183
dc.description.abstractWith the emergence of cell transplant as an attractive treatment modality for various diseases, there is a parallel need to track the fate of these cells to assess their therapeutic effectiveness. Here, we report the use of upconversion fluorescent nanoparticles, silica/NaYF4:Yb,Er, to dynamically track live myoblast cells in vitro and in a living mouse model of cryoinjured hind limb. Nanoparticles loaded into cells were confirmed for its intracellular uptake by confocal imaging, spectrophotometry and inductively coupled plasma analysis. Loaded nanoparticles demonstrated absolute resistance to photobleaching and were applied for dynamic imaging to real time track in vitro cell migratory activity for a continuous 5 h duration using a time-lapse confocal microscope. Direct observation on the direction, speed and cell-cell interaction of migrating cells was clearly visualized. In vivo confocal imaging of nanoparticle-loaded cells intravenously injected into a mouse tail vein showed them flowing in the ear blood vessels. Nanoparticle-loaded cells were also unambiguously identified with superior contrast against a negligible background at least 1300 μm deep in a fully vascularized living tissue upon intramuscular injection. Spatiotemporal migratory activity of the transplanted cells within the three-dimensional living tissue was captured for at least 7 days post-delivery. Direct in vivo visualization of cell dynamics in the native tissue was unobtrusively followed over a 4 h time course and revealed subtle migratory activity of the transplanted cells. With these unique optical properties, we present silica/NaYF4:Yb,Er nanoparticles as a new fluorescent live cell tracker probe for superior in vitro and in vivo dynamic imaging. © 2009 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2009.05.062
dc.sourceScopus
dc.subjectConfocal microscopy
dc.subjectNanoparticle
dc.subjectStem cell
dc.subjectTransplantation
dc.typeArticle
dc.contributor.departmentSURGERY
dc.contributor.departmentNATIONAL UNIVERSITY MEDICAL INSTITUTES
dc.contributor.departmentDIVISION OF BIOENGINEERING
dc.contributor.departmentPHARMACY
dc.description.doi10.1016/j.biomaterials.2009.05.062
dc.description.sourcetitleBiomaterials
dc.description.volume30
dc.description.issue28
dc.description.page5104-5113
dc.description.codenBIMAD
dc.identifier.isiut000269459900028
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