Please use this identifier to cite or link to this item: https://doi.org/10.1016/0378-4347(95)00468-8
DC FieldValue
dc.titleSimplified method for measuring urinary leukotriene E4
dc.contributor.authorQiu, D.W.
dc.contributor.authorHui, K.P.
dc.contributor.authorLim, T.K.
dc.contributor.authorTan, W.C.
dc.contributor.authorLee, C.W.
dc.date.accessioned2011-12-07T08:17:07Z
dc.date.available2011-12-07T08:17:07Z
dc.date.issued1996
dc.identifier.citationQiu, D.W., Hui, K.P., Lim, T.K., Tan, W.C., Lee, C.W. (1996). Simplified method for measuring urinary leukotriene E4. Journal of Chromatography B: Biomedical Applications 677 (1) : 152-155. ScholarBank@NUS Repository. https://doi.org/10.1016/0378-4347(95)00468-8
dc.identifier.issn15726495
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/29742
dc.description.abstractThe conventional method for measuring urinary leukotriene E4 (LTE4) is by reversed-phase high-performance liquid chromatography (RP-HPLC), followed by radioimmunoassay (RIA) or enzyme immunoassay (EIA). We measured urinary LTE4 levels by two methods, HPLC with EIA and EIA alone after initial crude extraction of urine using an octadecyl reversed-phase extraction cartridge (Sep-Pak). Ninety-three urine samples from normal subjects and patients with bronchial asthma and adult respiratory distress syndrome were tested. The results showed that urinary LTE4 levels measured by EIA significantly correlated with those measured by HPLC plus EIA in the three groups (r=0.88, 0.85, 0.68). The absolute values of urinary LTE4 measured by EIA without HPLC purification were higher than by EIA with HPLC purification. This suggests that HPLC may not be necessary for routine urinary LTE4 quantitation in different clinical situations.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0378-4347(95)00468-8
dc.sourceScopus
dc.subjectLeukotrienes
dc.typeArticle
dc.contributor.departmentMEDICINE
dc.contributor.departmentPHYSIOLOGY
dc.description.doi10.1016/0378-4347(95)00468-8
dc.description.sourcetitleJournal of Chromatography B: Biomedical Applications
dc.description.volume677
dc.description.issue1
dc.description.page152-155
dc.description.codenJCBBE
dc.identifier.isiutA1996TZ69600017
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