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Title: Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance
Authors: Lu, H.-F.
Lim, W.S.
Wang, J.
Tang, Z.-Q.
Zhang, P.-C.
Leong, K.W.
Mao, H.-Q. 
Chia, S.M. 
Yu, H. 
Keywords: Bioartificial liver assist device
Galactose immobilization
Hepatocyte culture
PVDF membrane
Issue Date: 2003
Citation: Lu, H.-F., Lim, W.S., Wang, J., Tang, Z.-Q., Zhang, P.-C., Leong, K.W., Mao, H.-Q., Chia, S.M., Yu, H. (2003). Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance. Biomaterials 24 (27) : 4893-4903. ScholarBank@NUS Repository.
Abstract: One of the major challenges in BLAD design is to develop functional substrates suitable for hepatocyte attachment and functional maintenance. In the present study, we designed a poly(vinylidene difluoride) (PVDF) surface coated with galactose-tethered Pluronic polymer. The galactose-derived Pluronic F68 (F68-Gal) was adsorbed on PVDF membrane through hydrophobic-hydrophobic interaction between PVDF and the polypropylene oxide segment in Pluronic. The galactose density on the modified PVDF surface increased with the concentration of the F68-Gal solution, reaching 15.4nmol galactosyl groups per cm2 when a 1mg/ml of F68-Gal solution was used. The adsorbed F68-Gal remained relatively stable in culture medium. Rat hepatocytes attachment efficiency on F68-Gal modified PVDF membrane was similar to that on collagen-coated surface. The attached hepatocytes on PVDF/F68-Gal membrane self-assembled into multi-cellular spheroids after 1 day of culture. These attached hepatocytes in spheroids exhibited higher cell functions such as albumin synthesis and P450 1A1 detoxification function compared to unmodified PVDF membrane and collagen-coated surface. These results suggest the potential of this galactose-immobilized PVDF membrane as a suitable substrate for hepatocyte culture. © 2003 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/S0142-9612(03)00404-6
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