Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0142-9612(03)00404-6
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dc.titleGalactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance
dc.contributor.authorLu, H.-F.
dc.contributor.authorLim, W.S.
dc.contributor.authorWang, J.
dc.contributor.authorTang, Z.-Q.
dc.contributor.authorZhang, P.-C.
dc.contributor.authorLeong, K.W.
dc.contributor.authorMao, H.-Q.
dc.contributor.authorChia, S.M.
dc.contributor.authorYu, H.
dc.date.accessioned2011-12-07T08:17:03Z
dc.date.available2011-12-07T08:17:03Z
dc.date.issued2003
dc.identifier.citationLu, H.-F., Lim, W.S., Wang, J., Tang, Z.-Q., Zhang, P.-C., Leong, K.W., Mao, H.-Q., Chia, S.M., Yu, H. (2003). Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance. Biomaterials 24 (27) : 4893-4903. ScholarBank@NUS Repository. https://doi.org/10.1016/S0142-9612(03)00404-6
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/29738
dc.description.abstractOne of the major challenges in BLAD design is to develop functional substrates suitable for hepatocyte attachment and functional maintenance. In the present study, we designed a poly(vinylidene difluoride) (PVDF) surface coated with galactose-tethered Pluronic polymer. The galactose-derived Pluronic F68 (F68-Gal) was adsorbed on PVDF membrane through hydrophobic-hydrophobic interaction between PVDF and the polypropylene oxide segment in Pluronic. The galactose density on the modified PVDF surface increased with the concentration of the F68-Gal solution, reaching 15.4nmol galactosyl groups per cm2 when a 1mg/ml of F68-Gal solution was used. The adsorbed F68-Gal remained relatively stable in culture medium. Rat hepatocytes attachment efficiency on F68-Gal modified PVDF membrane was similar to that on collagen-coated surface. The attached hepatocytes on PVDF/F68-Gal membrane self-assembled into multi-cellular spheroids after 1 day of culture. These attached hepatocytes in spheroids exhibited higher cell functions such as albumin synthesis and P450 1A1 detoxification function compared to unmodified PVDF membrane and collagen-coated surface. These results suggest the potential of this galactose-immobilized PVDF membrane as a suitable substrate for hepatocyte culture. © 2003 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0142-9612(03)00404-6
dc.sourceScopus
dc.subjectBioartificial liver assist device
dc.subjectGalactose immobilization
dc.subjectHepatocyte culture
dc.subjectPVDF membrane
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentMATERIALS SCIENCE
dc.description.doi10.1016/S0142-9612(03)00404-6
dc.description.sourcetitleBiomaterials
dc.description.volume24
dc.description.issue27
dc.description.page4893-4903
dc.description.codenBIMAD
dc.identifier.isiut000186267600001
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