Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0304-3835(98)00021-4
DC FieldValue
dc.titleCaspase proteases mediate apoptosis induced by anticancer agent preactivated MC540 in human tumor cell lines
dc.contributor.authorPervaiz, S.
dc.contributor.authorHirpara, J.L.
dc.contributor.authorClement, M.-V.
dc.date.accessioned2011-12-07T08:16:49Z
dc.date.available2011-12-07T08:16:49Z
dc.date.issued1998
dc.identifier.citationPervaiz, S., Hirpara, J.L., Clement, M.-V. (1998). Caspase proteases mediate apoptosis induced by anticancer agent preactivated MC540 in human tumor cell lines. Cancer Letters 128 (1) : 11-22. ScholarBank@NUS Repository. https://doi.org/10.1016/S0304-3835(98)00021-4
dc.identifier.issn03043835
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/29719
dc.description.abstractThe molecular events involved in tumor cell death induced by novel photoproducts of merocyanine 540 (pMC540) are poorly understood. Using HL60 leukemia and M14 melanoma cell lines we investigated the role of the apoptotic pathway in pMC540-mediated cell death. Tumor cells exposed to pMC540 showed cell size shrinkage and an increase in the sub-diploid DNA content. A loss of membrane phospholipid asymmetry associated with apoptosis was induced by pMC540 in both tumor cell lines as evidenced by the externalization of phosphatidylserine. A dose-dependent increase in caspase-3 protease activity suppressed by the tetrapeptide inhibitor DEVD-CHO was observed in both cell lines. Western blot analysis of poly (ADP-ribose) polymerase, a caspase substrate, showed the classical cleavage pattern (116 to 89 kDa) associated with apoptosis in pMC540-treated cell lysates. Furthermore, caspase inhibition blocked the externalization of membrane PS, indicating that the loss of membrane phospholipid asymmetry is a downstream event of caspase activation. These findings demonstrate that tumor cell death induced by pMC540 is mediated by caspase proteases.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0304-3835(98)00021-4
dc.sourceScopus
dc.subjectApoptosis
dc.subjectCaspases
dc.subjectPARP cleavage
dc.subjectPhosphatidylserine
dc.subjectPreactivated MC540
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentNATIONAL UNIVERSITY MEDICAL INSTITUTES
dc.description.doi10.1016/S0304-3835(98)00021-4
dc.description.sourcetitleCancer Letters
dc.description.volume128
dc.description.issue1
dc.description.page11-22
dc.description.codenCALED
dc.identifier.isiut000074123000002
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

15
checked on Dec 4, 2020

WEB OF SCIENCETM
Citations

23
checked on Nov 25, 2020

Page view(s)

206
checked on Dec 2, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.