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Title: Encapsulating live cells with water-soluble chitosan in physiological conditions
Authors: Zhu, J.-H. 
Yu, H. 
Wang, X.-W. 
Ng, S.
Ho, H.-T.
Lao, X.-J.
Quek, C.-H.
Keywords: Diffusion properties
Half N-acetylated chitosan
Hepatocyte functions
Mechanical stability
Issue Date: 2005
Citation: Zhu, J.-H., Yu, H., Wang, X.-W., Ng, S., Ho, H.-T., Lao, X.-J., Quek, C.-H. (2005). Encapsulating live cells with water-soluble chitosan in physiological conditions. Journal of Biotechnology 117 (4) : 355-365. ScholarBank@NUS Repository.
Abstract: A new class of microcapsules was prepared under physiological conditions by polyelectrolyte complexation between two oppositely-charged, water-soluble polymers. The microcapsules consisted of an inner core of half N-acetylated chitosan and an outer shell of methacrylic acid (MAA) (20.4%)-hydroxyethyl methacrylate (HEMA) (27.4%)-methyl methacrylate (MMA) (52.2%) (MAA-HEMA-MMA) terpolymer. Both 400 and 150 kDa half N-acetylated chitosans maintained good water solubility and supplied enough protonated amino groups to coacervate with terpolymer at pH 7.0-7.4, in contrast to other chitosan-based microcapsules which must be prepared at pH <6.5. The viscosity of half N-acetylated chitosan solutions between 80 and 3000 cPa s allowed the formation of microcapsules with spherical shape. Molar mass, pH and concentration of half N-acetylated chitosan, and reaction time, influenced the morphology, thickness and porosity of the microcapsules. Microcapsules formed with high concentration of half N-acetylated chitosan exhibited improved mechanical stability, whereas microcapsules formed with low concentration of half N-acetylated chitosan exhibited good permeability. This 3D microenvironment has been configured to cultivate sensitive anchorage-dependent cells such as hepatocytes to maintain high level of functions. © 2005 Elsevier B.V. All rights reserved.
Source Title: Journal of Biotechnology
ISSN: 01681656
DOI: 10.1016/j.jbiotec.2005.03.011
Appears in Collections:Staff Publications

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