Please use this identifier to cite or link to this item:
Title: Encapsulating live cells with water-soluble chitosan in physiological conditions
Authors: Zhu, J.-H. 
Yu, H. 
Wang, X.-W. 
Ng, S.
Ho, H.-T.
Lao, X.-J.
Quek, C.-H.
Keywords: Diffusion properties
Half N-acetylated chitosan
Hepatocyte functions
Mechanical stability
Issue Date: 2005
Citation: Zhu, J.-H., Yu, H., Wang, X.-W., Ng, S., Ho, H.-T., Lao, X.-J., Quek, C.-H. (2005). Encapsulating live cells with water-soluble chitosan in physiological conditions. Journal of Biotechnology 117 (4) : 355-365. ScholarBank@NUS Repository.
Abstract: A new class of microcapsules was prepared under physiological conditions by polyelectrolyte complexation between two oppositely-charged, water-soluble polymers. The microcapsules consisted of an inner core of half N-acetylated chitosan and an outer shell of methacrylic acid (MAA) (20.4%)-hydroxyethyl methacrylate (HEMA) (27.4%)-methyl methacrylate (MMA) (52.2%) (MAA-HEMA-MMA) terpolymer. Both 400 and 150 kDa half N-acetylated chitosans maintained good water solubility and supplied enough protonated amino groups to coacervate with terpolymer at pH 7.0-7.4, in contrast to other chitosan-based microcapsules which must be prepared at pH <6.5. The viscosity of half N-acetylated chitosan solutions between 80 and 3000 cPa s allowed the formation of microcapsules with spherical shape. Molar mass, pH and concentration of half N-acetylated chitosan, and reaction time, influenced the morphology, thickness and porosity of the microcapsules. Microcapsules formed with high concentration of half N-acetylated chitosan exhibited improved mechanical stability, whereas microcapsules formed with low concentration of half N-acetylated chitosan exhibited good permeability. This 3D microenvironment has been configured to cultivate sensitive anchorage-dependent cells such as hepatocytes to maintain high level of functions. © 2005 Elsevier B.V. All rights reserved.
Source Title: Journal of Biotechnology
ISSN: 01681656
DOI: 10.1016/j.jbiotec.2005.03.011
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Mar 16, 2023


checked on Mar 8, 2023

Page view(s)

checked on Mar 16, 2023

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.