Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0006-2952(01)00831-0
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dc.titleThe signalling pathway for BCG-induced interleukin-6 production in human bladder cancer cells
dc.contributor.authorZhang, Y.
dc.contributor.authorYap, L.L.
dc.contributor.authorKhoo, H.E.
dc.contributor.authorMahendran, R.
dc.contributor.authorEsuvaranathan, K.
dc.date.accessioned2011-11-30T06:34:12Z
dc.date.available2011-11-30T06:34:12Z
dc.date.issued2002
dc.identifier.citationZhang, Y., Yap, L.L., Khoo, H.E., Mahendran, R., Esuvaranathan, K. (2002). The signalling pathway for BCG-induced interleukin-6 production in human bladder cancer cells. Biochemical Pharmacology 63 (2) : 273-282. ScholarBank@NUS Repository. https://doi.org/10.1016/S0006-2952(01)00831-0
dc.identifier.issn00062952
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/29455
dc.description.abstractIntravesical bacillus Calmette-Guérin (BCG) is currently the therapy of choice for superficial bladder cancer with a 60-70% response rate. Induction of cytokine production (e.g. IL-6, etc.) by BCG has been found in patient's urine in vivo as well as bladder cancer cell lines. However, the signalling mechanisms are still unclear. In this study, we investigated the effect of BCG on cAMP production and its role in regulating interleukin-6 expression in the human bladder cancer cell line, MGH. After 1hr exposure to BCG, IL-6 gene expression in MGH cells increased by 2.5-3-fold and cAMP production increased by 8-10-fold in a time- and dose-dependent manner. BCG-induced cAMP production was inhibited by both antifibronectin antibody and an adenylate cyclase inhibitor, SQ22536 in a dose-dependent way. In the presence of SQ22536, IL-6 expression in MGH cells was also greatly reduced. Furthermore, cAMP-dependent kinase inhibitors H7 and HA1004 also inhibited BCG-induced IL-6 expression in MGH, with HA1004 being much less effective than H7. Thus, BCG induces cAMP production and may regulate interleukin-6 expression partially via a cAMP-dependent pathway in human bladder cancer cells. © 2002 Elsevier Science Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0006-2952(01)00831-0
dc.sourceScopus
dc.subjectBacillus Calmette-Guérin
dc.subjectcAMP
dc.subjectHuman bladder cancer cells
dc.subjectInterleukin-6
dc.typeArticle
dc.contributor.departmentSURGERY
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/S0006-2952(01)00831-0
dc.description.sourcetitleBiochemical Pharmacology
dc.description.volume63
dc.description.issue2
dc.description.page273-282
dc.description.codenBCPCA
dc.identifier.isiut000174556200023
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