Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.humimm.2005.01.017
DC FieldValue
dc.titleIdentification of CD8+T-cell epitopes specific for immediate-early transactivator Rta of Epstein-Barr virus
dc.contributor.authorYu, H.
dc.contributor.authorChan, S.
dc.contributor.authorSrinivasan, N.
dc.contributor.authorRen, E.
dc.date.accessioned2011-11-29T09:15:40Z
dc.date.available2011-11-29T09:15:40Z
dc.date.issued2005
dc.identifier.citationYu, H., Chan, S., Srinivasan, N., Ren, E. (2005). Identification of CD8+T-cell epitopes specific for immediate-early transactivator Rta of Epstein-Barr virus. Human Immunology 66 (5) : 483-493. ScholarBank@NUS Repository. https://doi.org/10.1016/j.humimm.2005.01.017
dc.identifier.issn01988859
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/29029
dc.description.abstractNasopharyngeal carcinoma (NPC) is a human epithelial tumor with a high incidence in Southern Chinese population, with contributions from Epstein-Barr virus (EBV), human leukocyte antigen (HLA), and environmental factors to its etiology. It has been shown previously that the recognition of immediate-early transactivator Rta of EBV by CD8+ T cells may have a significant impact on controlling EBV and, indirectly, NPC. The current study used two computer-aided prediction methods and competition-based HLA-peptide binding assays to screen for HLA B2704/B4601/B5801 restricted T-cell epitopes derived from Rta. HLA tetrameric complexes containing these potential T-cell epitopes were synthesized. Rta-specific CD8+ T-cell responses in healthy virus carriers were then defined by these tetramers and IFN-γ ELISPOT assays. We clearly demonstrated that healthy virus carriers have detectable Rta-specific CD8+ T cells restricted by B2704 in the circulation. However, there were no B4601/B5801 tetramer-reactive T cells specific for Rta in the peripheral blood of matched/mismatched donors. On the other hand, B4601 tetramers containing the computer-predicted B4601 binder EBNA3A (318-326) showed detectable tetramer-reactive T cells in the circulation of healthy virus carriers. topes also elicited IFN-γ responses as detected by ELISPOT. © American Society for Histocompatibility and Immunogenetics, 2005. Published by Elsevier Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.humimm.2005.01.017
dc.sourceScopus
dc.subjectEBV
dc.subjectEpitopes
dc.subjectHLA
dc.subjectT lymphocytes
dc.subjectTumor immunology
dc.typeArticle
dc.contributor.departmentWHO IMMUNOLOGY RESEARCH & TRAINING CTR
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.humimm.2005.01.017
dc.description.sourcetitleHuman Immunology
dc.description.volume66
dc.description.issue5
dc.description.page483-493
dc.description.codenHUIMD
dc.identifier.isiut000230042200005
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