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Title: Identification of CD8+T-cell epitopes specific for immediate-early transactivator Rta of Epstein-Barr virus
Authors: Yu, H. 
Chan, S. 
Srinivasan, N.
Ren, E. 
Keywords: EBV
T lymphocytes
Tumor immunology
Issue Date: 2005
Citation: Yu, H., Chan, S., Srinivasan, N., Ren, E. (2005). Identification of CD8+T-cell epitopes specific for immediate-early transactivator Rta of Epstein-Barr virus. Human Immunology 66 (5) : 483-493. ScholarBank@NUS Repository.
Abstract: Nasopharyngeal carcinoma (NPC) is a human epithelial tumor with a high incidence in Southern Chinese population, with contributions from Epstein-Barr virus (EBV), human leukocyte antigen (HLA), and environmental factors to its etiology. It has been shown previously that the recognition of immediate-early transactivator Rta of EBV by CD8+ T cells may have a significant impact on controlling EBV and, indirectly, NPC. The current study used two computer-aided prediction methods and competition-based HLA-peptide binding assays to screen for HLA B2704/B4601/B5801 restricted T-cell epitopes derived from Rta. HLA tetrameric complexes containing these potential T-cell epitopes were synthesized. Rta-specific CD8+ T-cell responses in healthy virus carriers were then defined by these tetramers and IFN-γ ELISPOT assays. We clearly demonstrated that healthy virus carriers have detectable Rta-specific CD8+ T cells restricted by B2704 in the circulation. However, there were no B4601/B5801 tetramer-reactive T cells specific for Rta in the peripheral blood of matched/mismatched donors. On the other hand, B4601 tetramers containing the computer-predicted B4601 binder EBNA3A (318-326) showed detectable tetramer-reactive T cells in the circulation of healthy virus carriers. topes also elicited IFN-γ responses as detected by ELISPOT. © American Society for Histocompatibility and Immunogenetics, 2005. Published by Elsevier Inc.
Source Title: Human Immunology
ISSN: 01988859
DOI: 10.1016/j.humimm.2005.01.017
Appears in Collections:Staff Publications

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