Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2008.06.052
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dc.titleRational design, solution conformation and identification of functional residues of the soluble and structured Nogo-54, which mimics Nogo-66 in inhibiting the CNS neurite outgrowth
dc.contributor.authorLi, M.
dc.contributor.authorLiao, X.
dc.contributor.authorQin, H.
dc.contributor.authorSong, J.
dc.contributor.authorLi, Y.
dc.contributor.authorXiao, Z.-c.
dc.contributor.authorLiu, J.
dc.date.accessioned2011-11-29T06:10:49Z
dc.date.available2011-11-29T06:10:49Z
dc.date.issued2008
dc.identifier.citationLi, M., Liao, X., Qin, H., Song, J., Li, Y., Xiao, Z.-c., Liu, J. (2008). Rational design, solution conformation and identification of functional residues of the soluble and structured Nogo-54, which mimics Nogo-66 in inhibiting the CNS neurite outgrowth. Biochemical and Biophysical Research Communications 373 (4) : 498-503. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2008.06.052
dc.identifier.issn0006291X
dc.identifier.issn10902104
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/28904
dc.description.abstractThe interaction between Nogo-66 and its receptor NgR represents a promising target for designing drugs to treat CNS axonal injury which often leads to permanent disability. Unfortunately, the isolated Nogo-66 is highly insoluble while its truncated form Nogo-40 is soluble but unstructured, thus retarding further characterization and application. Here, we rationally design another soluble form Nogo-54. CD and NMR characterization reveals that Nogo-54 is structured, and importantly, is able to mimic Nogo-66 in inhibiting neurite outgrowth. Strikingly, mutating its C-terminal four residues (Lys50, Glu51, Arg53, and Arg54) leads to a mutant Nogo-54m which has no dramatic structural change but whose inhibitory activity is completely abolished. This strongly suggests that the four charged residues contribute significantly to the inhibitory action of Nogo-66. Furthermore, our study also provides a soluble and structured mimic as well as a possible antagonist for Nogo-66 which may hold promising potential for various medical applications. © 2008 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2008.06.052
dc.sourceScopus
dc.subjectAntagonist design
dc.subjectCircular dichroism
dc.subjectCNS injury
dc.subjectNeurite outgrowth
dc.subjectNMR spectroscopy
dc.subjectNogo
dc.subjectNogo-66 receptor
dc.subjectSolution conformation
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.bbrc.2008.06.052
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume373
dc.description.issue4
dc.description.page498-503
dc.description.codenBBRCA
dc.identifier.isiut000258208500009
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