Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/28900
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dc.titlePTEN is recruited to specific microdomains of the plasma membrane during lactacystin-induced neuronal apoptosis
dc.contributor.authorChoy, M.S.
dc.contributor.authorCheung, N.S.
dc.contributor.authorBay, B.H.
dc.contributor.authorCheng, H.-C.
dc.date.accessioned2011-11-29T06:10:47Z
dc.date.available2011-11-29T06:10:47Z
dc.date.issued2006
dc.identifier.citationChoy, M.S., Cheung, N.S., Bay, B.H., Cheng, H.-C. (2006). PTEN is recruited to specific microdomains of the plasma membrane during lactacystin-induced neuronal apoptosis. Neuroscience Letters 405 (1-2) : 120-125. ScholarBank@NUS Repository.
dc.identifier.issn03043940
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/28900
dc.description.abstractThe tumor suppressor PTEN (phosphatase and tensin homolog deleted from chromosome 10) is a novel phosphatase displaying both protein and lipid phosphatase activities. In the central nervous system, PTEN plays an important role in the regulation of cell growth, differentiation and death. The tumor suppressor function of PTEN is attributed to its phospholipid phosphatase activity that dephosphorylates the plasma membrane phosphatidylinositol-(3,4,5)-triphosphate (PtdIns(3,4,5)P3). Since PTEN is normally localized in the cytosol, it needs to be targeted to the plasma membrane to dephosphorylate PtdIns(3,4,5)P3. We previously demonstrated that lactacystin-induced apoptosis of culture cortical neuron is associated with: (i) cleavage of PTEN (55 kDa) to a 50 kDa truncated form and (ii) accumulation of PTEN and all the truncated PTEN in a detergent-insoluble membrane fraction of the neuronal cells. Herein we demonstrate that a caspase-3 inhibitor suppresses cleavage of PTEN to the 50 kDa truncated form in culture cortical neurons in response to lactacystin treatment. Using immunogold transmission electron microscopy, we examined the distribution of PTEN in plasma membrane sheets stripped from cultured cortical neurons with and without treatment of lactacystin. Our results demonstrate that lactacystin treatment induces accumulation of PTEN to the inner surface of the plasma membrane sheets of the neuronal cells. Taken together, our data suggest that caspase-3-like proteases are involved in the conversion of PTEN to a 50-kDa truncated form and that PTEN and its truncated form accumulate at specific microdomains of the plasma membrane in neuronal cells undergoing apoptosis. © 2006 Elsevier Ireland Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.neulet.2006.06.037
dc.sourceScopus
dc.subjectApoptosis
dc.subjectCortical neurons
dc.subjectImmunogold TEM
dc.subjectLactacystin
dc.subjectLipid raft
dc.subjectPTEN
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.departmentANATOMY
dc.description.sourcetitleNeuroscience Letters
dc.description.volume405
dc.description.issue1-2
dc.description.page120-125
dc.description.codenNELED
dc.identifier.isiut000240169500024
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