Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.taap.2005.04.010
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dc.titleBroccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells
dc.contributor.authorRose, P.
dc.contributor.authorWhiteman, M.
dc.contributor.authorHuang, Q.
dc.contributor.authorOng, C.N.
dc.date.accessioned2011-11-29T06:09:41Z
dc.date.available2011-11-29T06:09:41Z
dc.date.issued2005
dc.identifier.citationRose, P., Whiteman, M., Huang, Q., Ong, C.N. (2005). Broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells. Toxicology and Applied Pharmacology 209 (2) : 105-113. ScholarBank@NUS Repository. https://doi.org/10.1016/j.taap.2005.04.010
dc.identifier.issn0041008X
dc.identifier.issn10960333
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/28805
dc.description.abstractA high dietary intake of cruciferous vegetables has been associated with a reduction in numerous human pathologies particularly cancer. In the current study, we examined the inhibitory effects of broccoli (Brassica oleracea var. italica) and watercress (Rorripa nasturtium aquaticum) extracts on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cancer cell invasion and matrix metalloproteinase-9 activity using human MDA-MB-231 breast cancer cells. Aberrant overexpression of matrix metalloproteinases, including metalloproteinase-9, is associated with increased invasive potential in cancer cell lines. Our results demonstrate that extracts of broccoli and Rorripa suppressed TPA-induced MMP-9 activity and invasiveness in a concentration dependant manner as determined by zymographic analysis. Furthermore, fractionation of individual extracts followed by liquid chromatography mass spectroscopy analysis (LC-MS) revealed that the inhibitory effects of each vegetable were associated with the presence of 4-methysulfinylbutyl (sulforaphane) and 7-methylsulphinylheptyl isothiocyanates. Taken together, our data indicate that isothiocyanates derived form broccoli and Rorripa inhibit metalloproteinase 9 activities and also suppress the invasive potential of human MDA-MB-231 breast cancer cells in vitro. The inhibitory effects observed in the current study may contribute to the suppression of carcinogenesis by diets high in cruciferous vegetables. © 2005 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.taap.2005.04.010
dc.sourceScopus
dc.subjectChemoprevention
dc.subjectCruciferous vegetables
dc.subjectInvasion
dc.subjectIsothiocyanate
dc.subjectMatrix metalloproteinase
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.departmentCOMMUNITY,OCCUPATIONAL & FAMILY MEDICINE
dc.description.doi10.1016/j.taap.2005.04.010
dc.description.sourcetitleToxicology and Applied Pharmacology
dc.description.volume209
dc.description.issue2
dc.description.page105-113
dc.description.codenTXAPA
dc.identifier.isiut000233732700002
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