Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2009.08.121
DC FieldValue
dc.titleUncoupling of oxidative phosphorylation by curcumin: Implication of its cellular mechanism of action
dc.contributor.authorLim, H.W.
dc.contributor.authorLim, H.Y.
dc.contributor.authorWong, K.P.
dc.date.accessioned2011-11-29T05:58:16Z
dc.date.available2011-11-29T05:58:16Z
dc.date.issued2009
dc.identifier.citationLim, H.W., Lim, H.Y., Wong, K.P. (2009). Uncoupling of oxidative phosphorylation by curcumin: Implication of its cellular mechanism of action. Biochemical and Biophysical Research Communications 389 (1) : 187-192. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2009.08.121
dc.identifier.issn0006291X
dc.identifier.issn10902104
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/28684
dc.description.abstractCurcumin is a phytochemical isolated from the rhizome of turmeric. Recent reports have shown curcumin to have antioxidant, anti-inflammatory and anti-tumor properties as well as affecting the 5′-AMP activated protein kinase (AMPK), mTOR and STAT-3 signaling pathways. We provide evidence that curcumin acts as an uncoupler. Well-established biochemical techniques were performed on isolated rat liver mitochondria in measuring oxygen consumption, F0F1-ATPase activity and ATP biosynthesis. Curcumin displays all the characteristics typical of classical uncouplers like fccP and 2,4-dinitrophenol. In addition, at concentrations higher than 50 μM, curcumin was found to inhibit mitochondrial respiration which is a characteristic feature of inhibitory uncouplers. As a protonophoric uncoupler and as an activator of F0F1-ATPase, curcumin causes a decrease in ATP biosynthesis in rat liver mitochondria. The resulting change in ATP:AMP could disrupt the phosphorylation status of the cell; this provides a possible mechanism for its activation of AMPK and its downstream mTOR and STAT-3 signaling. © 2009 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2009.08.121
dc.sourceScopus
dc.subject6-Ketocholestanol
dc.subjectCurcumin
dc.subjectF0F1-ATPase
dc.subjectProtonophore
dc.subjectRespiratory inhibitor
dc.subjectUncoupler
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/j.bbrc.2009.08.121
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume389
dc.description.issue1
dc.description.page187-192
dc.description.codenBBRCA
dc.identifier.isiut000274534900035
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

36
checked on Aug 21, 2019

WEB OF SCIENCETM
Citations

36
checked on Aug 21, 2019

Page view(s)

175
checked on Aug 19, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.