Please use this identifier to cite or link to this item:
Title: Evidence that selective changes in the lipid composition of raft-membranes occur during respiratory syncytial virus infection
Authors: Yeo, D.S.-Y.
Ying, L.
Sutejo, R.
Sugrue, R.J.
Chan, R.
Wenk, M.R. 
Brown, G.
Aitken, J.
Tan, B.-H.
Keywords: Lipid-raft
Respiratory syncytial virus
Virus assembly
Issue Date: 2009
Citation: Yeo, D.S.-Y., Ying, L., Sutejo, R., Sugrue, R.J., Chan, R., Wenk, M.R., Brown, G., Aitken, J., Tan, B.-H. (2009). Evidence that selective changes in the lipid composition of raft-membranes occur during respiratory syncytial virus infection. Virology 386 (1) : 168-182. ScholarBank@NUS Repository.
Abstract: We examined the structure of lipid-raft membranes in respiratory syncytial virus infected cells. Cholesterol depletion studies using methyl-β-cyclodextrin suggested that membrane cholesterol was required for virus filament formation, but not inclusion bodies. In addition, virus filament formation coincided with elevated 3-hydroxy-3-methylglutaryl-coenzyme A reductase expression, suggesting an increase in requirement for endogenous cholesterol synthesis during virus assembly. Lipid raft membranes were examined by mass spectrometry, which suggested that virus infection induced subtle changes in the lipid composition of these membrane structures. This analysis revealed increased levels of raft-associated phosphatidylinositol (PI) and phosphorylated PI during RSV infection, which correlated with the appearance of phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-triphosphate (PIP3) within virus inclusion bodies, and inhibiting the synthesis of PIP3 impaired the formation of progeny virus. Collectively, our analysis suggests that RSV infection induces specific changes in the composition of raft-associated lipids, and that these changes play an important role in virus maturation. © 2008 Elsevier Inc. All rights reserved.
Source Title: Virology
ISSN: 00426822
DOI: 10.1016/j.virol.2008.12.017
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jan 27, 2023


checked on Jan 18, 2023

Page view(s)

checked on Jan 26, 2023

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.