Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2009.08.069
DC FieldValue
dc.titleArtefacts in cell culture: Pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media
dc.contributor.authorLong, L.H.
dc.contributor.authorHalliwell, B.
dc.date.accessioned2011-11-23T02:25:40Z
dc.date.available2011-11-23T02:25:40Z
dc.date.issued2009
dc.identifier.citationLong, L.H., Halliwell, B. (2009). Artefacts in cell culture: Pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media. Biochemical and Biophysical Research Communications 388 (4) : 700-704. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2009.08.069
dc.identifier.issn0006291X
dc.identifier.issn10902104
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/28508
dc.description.abstractAscorbate and several phenolic compounds readily oxidise in cell culture media to generate hydrogen peroxide. However, media containing pyruvate showed much less H2O2 production, apparently because pyruvate can scavenge H2O2 in the medium. Researchers must be aware that compounds under test can sometimes readily oxidise in cell culture media, that this might not be detected by measurement of H2O2 if the media contain pyruvate, and that pyruvate can be substantially depleted in the media as a result. © 2009 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2009.08.069
dc.sourceScopus
dc.subjectAscorbate
dc.subjectCell culture
dc.subjectDulbecco's modified Eagle's medium
dc.subjectEpigallocatechin gallate
dc.subjectHydrogen peroxide
dc.subjectPyruvate
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/j.bbrc.2009.08.069
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume388
dc.description.issue4
dc.description.page700-704
dc.description.codenBBRCA
dc.identifier.isiut000274534700015
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